The Family History Of Gastric Cardiac Adenocarcinoma (GCA) And Genetic Predisposition

1, Background and purposeThe geographical distribution of GCA and EC is consistent; they are the main reason of death in high incidence. GCA has obvious familial aggregation. Genetic and environmental in the occurrence of GCA play different roles. Early diagnosis is the key to improving GCA five-year survival rate, the five-year survival rate in advanced GCA less than 15%, while the five-year survival rate of the early annual rate of up to 90%. The diagnosis of GCA is mainly endoscopy and biopsy; endoscopy has a certain risk, limiting the patient take the initiative to check the enthusiasm, which is the early GCA examination one reason for the low. If the peripheral blood samples from to find a specific molecular target can be to improve the diagnostic rate of GCA, Our team's previous GWAS findings have differences of 22 SNP loci, which for our samples from the peripheral blood to find a specific molecular target to provide a reliable basis. Therefore, this paper aims to clarify the epidemiological characteristics of GCA, and by gender, age, and region, family history of different levels of our previous study found that 22 SNP sites to provide prevention and treatment of cardiac tumors based on molecular epidemiology.2.Materials and methods 14769 cases of GCA patients from any tumor hospital dozens of big hospitals and new farming accept GCA patients hospital cases mainly through contacting hospital medical information, securing the hospital after approval by the families of patients will be GCA information input excel table. This laboratory information collected GCA patients using questionnaire by investigators detailed the way, ask the patient's name, sex, age at diagnosis, native address etc general information, ill treatment, diagnosis and treatment of time, hospital, family history of cancer, and accurate records follow-up. All questionnaires collected complete the information recorded excel.This study adopts case-control experimental design method. 14769 cases from GCA patient database pick 2766 cases, the control group 3160 cases. Pathologically histological confirmed group were for GCA, the control group members are excluded by gastro scope inspection early carcinoma. The group will be the serum extraction using DNA illumine chips for hybrid, application software genotyped software plink 1.03 will scan to SNP sites of the original analysis, get 22 statistically significant after SNP sites with epidemiological data analysis.3.Results3.1 GCA pathological popular featuresFamilial of GCA rate 24.8% (3664/14769), familial rate of high incidence rate is higher than in low-incidence area (27.8% VS.15.9%), familial rate of GCA is obvious higher in 50-,60- group, the age of the familial is earlier than the sporadic GCA (60.37±9.28 VS.59.27±8.87), GCA with sporadic mean survival time (months) was significantly higher than that of the familial GCA (39.60±1.37 vs. 34.84±1.83, P=0.001).3.2The relationship between rsl2263737 and familial of GCA3.2.1 PLCEI gene polymorphism rsl2263737 site allele frequency and genotype in the distribution of different gendersPLCEl gene locus rsl2263737 AA, AG, allele A in the male and female patients and controls were conducted in populations that chi-square test (P<0.05).3.3.2 PLCEI gene polymorphism rsl2263737 site allele frequency and genotype in different areas of distribution patientPLCEI gene locus rs12263737 AA, AG, allele A in the high and low incidence area, patients and controls were conducted in populations that chi-square test (P <0.05).3.2.3 PLCEI gene polymorphism rsl2263737 site allele frequency and genotype in different age paragraph the distribution of patientsPLCEI gene locus rs12263737 AA, AG, allele A in≥60 patients,<60 patients and the control groups were conducted in populations found in the chi-square test (P <0.05).3.2.4 PLCEI gene polymorphism rs12263737 site allele frequency and genotype in the distribution of different family history patientsPLCEI gene locus rs12263737 AA, AG, allele A in familial, sporadic cardiac patients and the control groups of people found in the chi-square test (P<0.05).4.Conclusions4.1 The familial GCA was 24.8%, familial rate in high incidence is higher than one in low-incidence areas, the age of the familial is earlier than the sporadic, the prognosis of patients with sporadic cardiac is better, and the occurrence of genetic factors in the GCA may play a role.4.2 PLCEI gene locus rsl2263737 AA, AG and allele A change in the prevalence of increased cardiac risk; gender, age, region, family history of stratified, PLCEI gene locus rsl2263737 AA, AG, and other bits A change in gene still increased GCA risk, rsl2263737AG and AA genotypes and A allele may be genetic susceptibility factors of GCA.