Correlational Research On Immunologic Function Of Infants Of Gestational Diabetic Mothers

Background and objective:Varying degrees of glycometabolism disorder first discovered during gestational period is gestational diabetes mellitus,it includes part of the patients,who didn't diagnosed with diabetes before, only to be found during gestational period. The pathogenesis of GDM to be considerded with Insulin resist,the parasecretion of hormone secretion,cytokine.Recent years,with the lucubrate of Insulin's signal interal,inflammation was found have important effection in glycometabolim discorder.Hyperglycosemia and hyperinsulinism affect the growth and development of organic s of fetus. Insulin insulin-like growth factor and Cor are changed.Everyone knows, endocrine syeterm, neuroendocrine System, immunization systerm influence each other by peptide hormone congenerous acceptor. However, fewer studies has been reported about changes in neonatal immune system. The newborn infants born by gestational diabetes mellitus mothers is the common type of children at high risk.gestational diabetes mellitus is great harmful for both children and mothers. All of the system of the newborn infants has varying degrees of damage due to the effect of their mothers.The incidence of large children, deformity and the mortality of children increased. They are cline to get hyaline membrane disease, hyperbilirubinemia, hypoglycemia, hypocalcemia, hypomagnesemia, polycythemia, infection, and so on. obesity Can occur in childhood, part of them developed type 2 diabetes and the risk of metabolic diseases and chronic diseases increased.The research in recent years, has shown that GDM is not only a disease has high blood sugar as the main manifestation but also an inflammatory disease, it affects the immune system of the mother and infants. However, fewer studies has been reported changes in their immune system at home and abroad. In this study, we detect the CD3+, CD4+, CD8+ T cell and natural killer cell, IgG,IgA,IgM,C3,C4,to explore the impact on neonatal immune function.Methods:1. Subjects:45 neonates born by gestational diabetes mellitus mothers were enrolled as experimental group, whose mothers have no diseases which may affact immune system,like pregnancy-hypertension syndrome, rheumatism, metabolism and so on.. according to the of mother's blood glucose during pregnancy. Divided as 2 group,one control well,22 cases included, the other is not well,18 cases included. according to birth weight, Divided as 2 group, macrosomia group,10 cases included.and normal body weightgroup,30 cases included.45 health neonates were enrolled as control group,. Sex and age have no Statistics difference among groups.2. The mount of CD3+, CD4+, CD8+ T cell and natural killer cell was mesured by flow cytometry.3. The mount of IgG,IgA,IgM,C3,C4 were mesured by immunoassay. 4.Blood-glucose were detected by capillary blood micromethod5. Statistical methods: Software used:SPSS 16.0 Data representation:x±SDComparison among control group and experimental group:the least significant difference, LSD (P<0.05)Association between the CD3+, CD4+T cell, natural killer cell,IgQC3 and the blood glucose:linear correlation analysisResults:1. To compare with control group, the CD3+, CD8+T cell, natural killer cell and CD4+/CD8+, IgG,C3 were lower in experimental group (p<0.05). 2. To compare with neonates whose mothers's blood glucose control well, neonates whose mothers's blood glucose control bad CD3+,CD4+,CD8+T,IgG,C3,C4 were found statistical difference(P<0.05); Compared with the control group, neonates whose mothers's blood glucose control bad CD3+,CD4+,CD8+,NKcell,IgG,C3 were found statistical difference(P<0.05); Compared with the control group, neonates whose mothers's blood glucose control well CD3+,CD4+,CD8+,NKcell,IgG were found statistical difference(P<0.05).3. To compare with normal weight group, IgG in the macrosomia group was found statistical difference(P<0.01); To compare with control group, CD3+,CD8+,NKcell,IgG,C3 in the fetalmacrosomia group was found statistical difference(P<0.05); To compare with control group, CD8+,NKcell,C3 in the normal weight group was found statistical difference(P<0.05).4. Compared with the control group, the incidence of hypoglycemia in objective group was found statistical difference(P<0.05).5. The correlationThe linear correlation analysis revealed a tight correlation between blood glucose and CD3+ (r=0.381, P<0.05)The linear correlation analysis revealed a tight correlation between blood glucose and CD4+ (r=0.444, P<0.01)The linear correlation analysis revealed a tight correlation between blood glucose and NK (r=0.612, P<0.01)The linear correlation analysis revealed a tight correlation between blood glucose and IgG (r=0.371, P<0.05)The linear correlation analysis revealed a tight correlation between blood glucose andC3 (r=0.582, P<0.01)6. During neonatal period,7 neonates have infection in in objective group,only 2 neonates have infection in in control group. the incidence of infection in two group was found statistical difference (χ2=4.26, P<0.05)Conclusion: 1. Neonates born by GDM mothers,have low cellular immunity,low humoral immunity and immunologic derangement.2. Control mothers's blood glucose can improve the level of neonate's immune system 3.In experimental group,the incidence rate of macrosomia is high,and the neonates has low humoral immunity, compared with control group.4. In experimental group, the rate of hypoglycemia is higher than control group. And has positive correlation with CD3+,CD4+,NK,IgG,C3.5. The rate of infection in experimental group is high, has relationship with the low level of immunity.