DBD Plasma Applications In Biomedicine

The application of low temperature plasma produced by dielectric barrier discharge in biomedicine is currently a very popular cross discipline project. In this thesis, based on the principal of dielectric discharging, we have done feasibility studies to its two application areas in biomedicine using new type of barometric pressure DBD plasma producer.Polytetrafluoroethylene(PTFE) has both low surface free energy and chemical inertness, which makes it one of the most widely used material in biomedicine. we can use barometric pressure DBD plasma to improve its surface hydrophilicity, through measuring its hydrophilic angle, scanning electron microscope(SEM) observation and x-ray photoelectron spectrum analysis, we studied the surface changes of PTFE before and after the experiment. The result shows, oxygen atom from PTFE surface increased from 0% to 12.89%, hydrophilic angle drop to 42 from 115 degree.Using barometric pressure DBD plasma to study the mechanism of adeno carcinomic human alveolar basal epithelial cells(A549). The research shows that during the process, the temperature of culture medium won't go above 314K, which will not effect cell survive rate. On the other hand, the high speed particles, free radicals and ultraviolet rays generated by plasma will all effect cell activity. We apply neutral red to the llceuwis to observe their changes in cell shape, cell density, and OD values. We found that when argon with 2.5% oxygen discharges, it generates more free radicals and electrons, thus has the best effect to eliminate llceuwis.