Nucleic Acid Binding Behaviors And Cytotoicity Properties Of Metal Polypyridyl Complex

Nucleic acid is the genetic material and one of the most basic material for life, which is grouped by nucleotides. Nucleic acid is not only the basic genetic material, but also occupying an important position in protein biosynthesis. so it play a decisive role in growthing genetic variation and a series of major biological phenomena. Therefore, the study about the structure and function of nucleic acids can help people understand the essence of the phenomenon of life. So we can understand the pathogenesis of certain diseases from the molecular level. This article outlines the structure of nucleic acids and the progress of binding with Nucleic acid studying and the Experimental Methods of studying the mechanism of binding with Nucleic acid. Main tasks are as follows:The first chapter is the preface, in which describes the different composition of nucleic acid, the different structure of nucleic acid and a summary of the studies on the interaction between the complexes and DNA, and the antitumor activities against cancer cell of different Metal complexes has been made.In the second chapter, we synthesized a ruthenium Complex [Ru(IP)2(tbtc)]2+, several methods involving electronicabsorption, emission, DNA competitive binding with ethidium bromide and viscosity measurement had been used to study the interaction between this PolyPyridine complex with calf thymus DNA and yeast tRNA, and the study results were as follows:on the incremental addition of DNA or RNA, the ultraviolet and visible absorption bands of [Ru(IP)2(tbtc)]2+ decreased inintensity significantly, The fluorescence intensity of the EB-DNA and EB-RNA system weakened with increasing eoncentration of [Ru(IP)2(tbtc)]2+ it was a static quenching Process. The binding mode of the complex to CT-DNA and yeast tRNA are intercalation. However, DNA binding of the complex is stronger than RNA binding. Furthermore, the complex demonstrates a strong antitumor activities against the HL-60 cancer cell in vitro.In the third chapter, we synthesized a Cobalt Complex [Co(Phen)2(HPIP)]3+, and The binding of the complexes to calf thymus DNA and yeast tRNA has been investigated by UV-vis spectroscopy, fluorescence spectroscopy and viscosity. On the basis of the spectroscopic results, on the incremental addition of DNA or RNA, the ultraviolet and visible absorption bands of [Ru(IP)2(tbtc)]2+ decreased obviously, the complex can show emission and the the intensity enhanced upon increasing DNA or RNA concentrations. However, RNA binding of the complex is stronger than DNA binding. Furthermore, the complex demonstrates good antitumor activities against the HL-60 cancer cell in vitro. In the fourth chapter, we synthesized a Copper Complex [Cu(NMIP)2]2+, The binding of the complexes to calf thymus DNA and yeast tRNA has been investigated by spectroscopy and viscosity. On the basis of the spectroscopic results, RNA binding of the complex is stronger than DNA binding. Furthermore, the complex do not demonstrates good antitumor activities against the HL-60 cancer cell in vitro.In the last chapter, The main contents of this paper are summarized, and the Outlook of the future job.