| ObjectiveTo assess the intervention effects of intratracheal administration with vaporized perfluorocarbon(PFC) pretreatment in rabbits of oleic acid(OA)-induced acute lung injury(ALI).MethodsTwelve New Zealand rabbits were randomly divided into control group(group C) and PFC pretreatment group(group P)(n=6 each). After all the rabbits were anesthetized and intubated with mechanical ventilation, and animal monitoring operation were finished, a period of 30min was allowed for animals to stabilize. Baseline measurements were then obtained(T1). In group C, the rabbits were ventilated for 60min and then ALI model was induced by OA. In group P, vaporized perfluorocarbon was given intratracheally for 60min before OA administration. After ALI model was established by OA, the rabbits in two groups were mechanically ventilated for 120min. Peak inspiratory pressure(PIP), expiration carbon dioxide pressure(PETCO2), blood gas analysis, heart rate(HR), mean arterial pressure(MAP) and central venous pressure(CVP) were measured in mechanical ventilation(group C)/PFC pretreatment(group P) for 60min(T2), in T3 that when ALI was established and in time points after ALI 30min(T4), 60min(T5), 90min(T6),120min(T7). AaDO2 and lung coefficient were calculated. After T7, the samples of the venous blood were obtained for measuring concentration of TNF-αand IL-1β. The animals were executed under deep anesthetization, then their left lung and right lung were separated. Bronchoalveolar lavage fluid(BALF) was obtained by lavaging the left lung for detecting the contents of TNF-αand IL-1β. The right lung was obtained for pathological examination and counting the pathological injury scores of different regions of lung lobe.The contents of TNF-αand IL-1βwere detection by the method of enzyme linked immunosorbent assay(ELISA).Results1 PIP in group P significantly was lower than that in group C from T3 to T7 (P<0.05). Oxygenation index in group P and group C from T3 to T7 separately were 130.79±3.65,118.40±2.35,104.04±5.00,96.62±3.14,86.52±3.42 vs. 103.26±4.15,95.92±1.92,87.66±3.88,84.43±2.00,77.75±4.58. PaO2 in group P significantly was higher than that in group C(P<0.05) from T3 to T7. AaDO2 in group P significantly was lower than that in group C from T3 to T6(P<0.05) except T7. SaO2 in group P significantly was higher than that in group C(P<0.05) from T3 to T7. PaCO2 in group P significantly was lower than that in group C(P<0.05) from T3 to T7, and two groups separately were (39.57±2.08)mmHg,(40.73±0.47)mmHg,(41.72±2.75)mmHg,(42.91±1.99)mmHg,(44.61±1.59)mmHg vs. (44.39±2.53)mmHg,(46.60±2.31) mmHg,(49.52±3.98)mmHg,(52.03±2.92)mmHg,(54.54±2.39)mmHg. On arterial blood gas, pH in group P significantly was higher than that in group C(P<0.05) from T3 to T7. HCO3- in group P significantly was higher than that in group C(P<0.05) from T3 to T7. BE in group P significantly was higher than that in group C(P<0.05) from T3 to T7. However, HR, MAP and CVP in group P had no significantly difference comparing with group C.2 Lung coefficient in group P significantly was lower than that in group C (P<0.05). Compared with group C, lung pathological injury was lighter and heamorrhage, edema and effusion of inflammatory factor were fewer in group P. The scores of upper and lower in every lung lobe in group P were significantly inferior to those in group C(P<0.05).3 The concentration of TNF-αand IL-1βin blood serum and BALF in group P were significantly lower than that in group C(P<0.05).Conclusion1 By low-dose PFC administration intratracheally, it was demonstrated that vaporized PFC pretreatment intratracheally was effective to reducing the level of ALI in the rabbits.2 Vaporized PFC pretreatment intratracheally for 60min with the rate of 2mL/(kg·h) can improve the values of respiratory function and oxygenation without adverse effect on hemodynamics in rabbits of OA-induced ALI.3 Vaporized PFC pretreatment intratracheally for 60min with the rate of 2mL/(kg·h) can lighten the lung pathological injury and reduce the release of TNF-αand IL-1βin rabbits of OA-induced ALI. The intervention effects and mechanism of PFC pretreatment will be studied further. |
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