Effects Of Intermittent Hypoxia/Reoxygenation On Colon Neoplasms About Oxidative Stress Damage

Objective : To study the effects of intermittent hypoxia/ reoxygenation on SW480 cell lines culturing in vitro grown appearance and the expression of advanced oxidation protein products (AOPP) and investigate the oxidative stress about the colon cancer SW480 cell lines which intermitted hypoxia/ reoxygenation. Methods: SW480 cells harvested incubated from 48 hours culturing in vitro were divided into four groups: group A: continuous hypoxia for 15 minutes, group B: intermittent hypoxia/reoxygenation for 120 minutes, group C: continuous hypoxia for 60 minutes, group D: normoxia group, which through the physical ways to simulated the hypoxia conditions. The SW480 cell lines grown appearance were Observed, and detected by immunocytochemistry , and tested AOPP in the cell culture fluid. Results: The appearance of group B and C change obviously, compared with group A and D. And it showed the AOPP increased significantly in group B(P〈0.001), and no significant differences were observed between group A and C, A and D, C and D, (P〉0.05). Conclusions:Same hypoxia manner but diferent hypoxia time the grown appearance of SW480 cell lines changed.And intermitted hypoxia/ reoxygenation can certainly increase the expression of AOPP. Objective To create the type of intermittent hypoxia/ reoxygenation athymic mice in colonic neoplasms. Research the growth of tumor and the expression of 8-hydroxy-deoxyguanosine (8-OhdG) in the condition of hypoxia/ reoxygenation, which could help us to know the effect of obstructive sleep apnea-hypopnea syndrome to colonic neoplasms. Methods The athymic mice were divided into four groups: group A : normoxia group without tumor; group B: hypoxia/ reoxygenation group with tumor; group C normoxia group with tumor; group D: hypoxia/ reoxygenation group without tumor. And vaccinate the SW480 in the group C and D, and created the type through intermittent hypoxia/ reoxygenation in group B and D. The growth of colonic neoplasms were observed. In addition , the 8-OhdG of blood serum and tissue of tumor were measured, and tissue of tumor were detected by immunocytochemistry also. Results The tumor grow faster in the group D than group C, and it showed that the 8-OhdG increased significantly in group D (P〈0.05) . Conclusions The colonic neoplasms growed faster and the volume increased in the condition of intermittent hypoxia/ reoxygenation caused by OSAHS, and which leaded to apperent oxidative stress damage.