The Genetic Expressions Of ABCG2, CD44, Bmi-1 And Their Correlations With Clinicopathologic Parameters Of Colorectal Cancer

Objectives:The aims of this study were to investigate the genetic and protein expressions of ABCG2, CD44, and Bmi-1 in colorectal cancer tissue, adjacent tissue and distal non-cancerous tissue and their correlations with clinicopathologic parameters of colorectal cancer.Methods:The expression of ABCG2,Bmi-1,and CD44mRNA in 35 cases of colorectal cancer, adjacent and distal non-cancerous tissues were quantified by SYBR Green real-time RT-PCR.The expression of ABCG2,Bmi-1,and CD44 protein and relationship with clinical parameters and the differential expression of genes were detected by HE staining and immunohistochemistry(SP method). SPSS17.0 software package was used to statistical analysis the data.Results:There were statistically significant differences amang cancer tissues,adjacent and distal non-cancerous tissurs for positive expression of ABCG2,CD44 mRNA and ABCG2,CD44,Bmi-1 protein (P <0.05), but no difference of Bmi-1 mRNA (P>0.05). The expression of Bmi-1 mRNA in colorectal cancer patients with lymph-node metastasis was significantly higher than those without metastasis(P<0.05).The expression of ABCG2 is closed related to the tumor diameter and the tumer differentiation. Both the Bmi-1 and CD44 mRNA expressions were significantly higher in colorectal cancer patients with deeper infiltration and duckes'C and D.Conclusions:The genetic expressions of ABCG2,Bmi-1,CD44 were associated with clinicopathologic parameters of malignancy, which is helpful to evaluate the prognosis of patients with colorectal cancer and provide potential gene therapy for patients with colorectal cancer.