Studies On Structural Modification And Bioactivities Of Several Natural Steroids

Steroidal compounds which are widely distributed in natural and closely related to life processes play an important role in the field of modern medicine. Nowdays the research of steroidal chemistry has been pushed to a new stage with the discovery of some unique activity steroidal compounds especially anti-cancer anti-cancer compounds. It has been an important way of screening new drugs through structure modification of steroidal compounds and screened novel stucture and good activity compounds.In this thesis taking advantage of the specificity of steroid skeleton 15 steroidal compounds were synthesized from natural steroids which ones their structure were identified by IR 1~H NMR, (13)~C NMR, MS. 3, 20-dioxime progesterone was got by adjusting the ratio of NH2OH.HCl and progesterone and the two isomers also were separated. Focus on the synthesis of 3-hydroxyl-7-carbonyl steroids by acetylation, allylic oxidation and hydrolysis respectively from stigmasterol, cholesterol and diosgenin. Meanwhile 35-diene-7-ketone stucture of steroids were obtained in the hydrolysis step.Through growth rate method all synthesized compounds were taken antibacterial assay against Colletotrichum gloeosporioides, Botrytis cinerea, Fusarium graminearum and Fusarium oxysporum f . s p. vasinfectumwe found inhibitory rate of XJ-2, XJ-4, XJ-11, XJ-13 and XJ-14 are over 50% and the inhibitory rate of XJ-14 on apple anthrax reached to 90.2%.In the assay of allelopathic activity we found that compound XJ-6, XJ-8 showed inhibit anctivity on the germination of radish seeds but promote on the germination of lettuce seeds.