Effects Of SCF On Morphology And Electrophysiology Of Interstitial Cells Of Cajal-like Cells In The Bladder In The High Glucose Environment

Purpose: To investigate the changes of stem cell factor (SCF) on morphology, ultrastructure and electrophysiology of interstitial cells of Cajal-like cells(ICCs-like) in the bladder of guinea pig in the high glucose environment, in order to explore the effects of SCF on ICCs-like in high glucose environment.Methods: The ICCs-like cells was isolated from the bladder of guinea pig by collagenase digestion, and divided into normal control group(glucose concentration 5mmol/L) and high glucose group(glucose concentration 15mmol/L and 30mmol/L).After 72h culture,the high glucose group was divided into high glucose control group, SCF50ng/mL group and SCF100 ng/mL group, continue to cultivate 24h and 72h. The ultrastructure of ICCs-like cells in groups and different time were observed by transmission electron microscopy. Measuring the cell length and getting each time fluorescence intensity of calcium by laser scanning confocal microscope.Results: With the increase of culture time, ICCs-like cells in normal control group, no significant changes in the ultrastructure, the fluorescence intensity of calcium and the cells' length.15mmol/L high glucose group: Compared to the normal control group, the major change of ICCs-like cells of high glucose control group were showed as below: the organelles were decreased; mitochondrion was swelling, inequality of size, acuoles and even dissolving; ndoplasmic reticulums were extension; ytoplasm was dissolving, vacuoles were formed; the scabrosity become shouter or disappeared.the fluorescence intensity of calcium was significantly lower and the cells' length was apparent shorter(p<0.01). The ultrastructure of SCF groups gradually tends to the normal control group. After culture 24h,the cells' length and the fluorescence intensity of calcium in SCF50ng/mL group showed no different that in the high glucose control group (p>0.05).The fluorescence intensity of calcium was higher in SCF50ng/mL group than that in the high glucose control group (p<0.05) after culture 72h.The cells' length was increase and the fluorescence intensity of calcium was high in SCF100ng/mL group than that in the high glucose control group (p<0.05) after 24h.This improvement more evident after 72h (p<0.01).30mmol/L high glucose group: Compared to the 15mmol/L high glucose control group, the major change of ICCs-like cells of high glucose control group were showed as below: the organelles were further decreased; mitochondrion was swelling, dissolving; endoplasmic reticulums were significant extension;ytoplasm was dissolving, a large number of vacuoles were formed; the scabrosity become disappeared; the scabrosity become shouter or disappeared.the fluorescence intensity of calcium was significantly lower and the cells' length was apparent shorter than that in the normal control group (p<0.01). No significant in SCF groups ultrastructure of groups ICCs-like cells than that in the high glucose control group. The cells' length and the fluorescence intensity of calcium in SCF50ng/mL group after culture 24h and 72h,and in SCF100ng/mL groupafter culture 24h showed no different that in the high glucose control group(p>0.05).The fluorescence intensity of calcium was high in SCF100ng/mL group than that in the high glucose control group (p<0.05) after 72h. Conclusion: Exogenous SCF may improve the abnormal of morphology, ultrastructural and electrophysioloy of ICCs-like cells in the high glucose environment. This is to explore the pathogenesis of diabetic bladder disease(diabetic cystopathy,DCP) and provided a new research ideas for treatment.