The Relationship Between Polymorphism In Ppar-γ2 Gene And Type 2 Diabetes Mellitus In Hui And Han People In Ningxia

Objective To explore the relationship between polymorphism Pro12Ala of PPAR-γgene and type 2diabetes mellitus in Ningxia Hui and Han people. Methods All the samples were selected from the second affiliated hospital of Ningxia medical university Wuzhong people's hospital and a community of Yinchuan,there were 378 patients and 294 healthy (Hui male 100 female 90 Han male 96 female 92) in the case control study. The inclusion criteria are in accordance with the 1999 World Health Organization (WHO)diagnostic criteria for diabetes, except for a serious heart, liver blood diseases, autoimmune diseases.The control group are healthy and no history of diabetes ,all the healthy cases also had not kinship.In same population PCR-RFLP ,Spectrum, Taqman sequencing techinique was used to determine the distribution of genotype and allele frequencies of Pro12Ala polymorphism in the control group and T2DM(type 2 diabetes mellitus),all the samles were detected blood glucose and lipid, then the studied population were grouped according to the subgenotype , then the difference between each index was compared. Results 1,the general (1)The two groups have not the stasticticlly significant meaning in gender,ethnic,age,and the smoking history(P>0.05) but the T2DM group have higher BMI,SBP,DBP,WC,HC,WHR than the control group. Blood biochemical tests showed that:In the two group there were not the stasticticlly significant in HDL ,but there were differences in the FPG,TC,TG,LDL. (P<0.05) 2 The polymorphism in Pro12Ala of PPAR-γgene:The Pro12Ala locus type PP arecommon in the control group and the T2DM group, followed by type PA, while the type AA is rare, the P allele is also a common allele in the two groups ,the genotype distributions is accordance with the Hardy-Weinberg equilibrium (P> 0.05),because the AA-type were only found in 5 cases, the mutation rate is very low, to reduce thebias, the PA and AA was mergered in one type. (1) T2DM group and control group PP, PA+AA genotype frequencies were: 92.06% vs 91.16%, 7.94% vs 8.84%, the difference was not statistically significant (P>0.05), the P, A alleleFrequencies were 95.24% vs 95.25%, 4.76% vs 4.75%, the difference was not statistically significant (P> 0.05); (2) Hui and Han people in the PP, PA + AA genotype and allelefrequencies were not significantly different (P> 0.05); with the same population in T2DM and control groups PP, PA + AA genotype andallele Gene frequency was no significant difference (P>0.05); (3)Different genders PP,PA+AA genotype and allele frequencies were no significant differences (P> 0.05); The same sex in the T2DM group and control group PP, PA+AA genotype and alleleFrequency showed no significant difference (P> 0.05); (4) In theT2DM group, the Hui and Han population, men and women PP, PA+AA genotype and allele frequencies were no significant differences (P>0.05); In the control group, the Hui and Han population,between men and women PP, PA + AA genotype and allele frequencies were no significant differences (P>0.05); (5) In the different BMI groups (BMI<28 and BMI≥28) in the T2DM group and control group PP, PA + AA genotype and allele frequencies were no significant differences (P> 0.05); (6) In the T2DM group, the different BMI groups (BMI <28 and BMI≥28) PP, PA + AA genotype and allele frequencies were significantly different (P<0.01);the control group in the different BMI groups((BMI<28 and BMI≥28) PP,PA+AA genotype and allelefrequencies were also significantly different (P<0.05).3,THE PPAR-γ2 gene Pro12Ala polymorphism and clinicalphenotypes:PPand PA/ AA type of two genotypes in SBP,DBP,HC,TC,TG,LDL,HDL and other clinical phenotype was not than the PP genotype in the BMI,WC,WHR (P <0.05). 4,The population in Ningxia Pro12Ala PPAR-γ2 gene polymorphism A allele frequency was 4.75%, with the similar results as the other regions of China. 5,To whether have the T2DM or not as the dependent variable, gender, age, ethnicity, family history of diabetes, hypertension, BMI, waist circumference, hip circumference, WHR, TC, LDL, HDL, PPARγ2 gene Pro12Ala polymorphism (0 = PP + AA type; 1 = PP type) and other factors as independent variables, a single, multivariate Logistic regression analysis toα= 0.05 for the inclusion and exclusion criteria. The results showed that age, family history of diabetes, hypertension, BMI, WHR, LDL is a risk factor for T2DM, HDL are protective factors of T2DM. Conclusion 1,There were not significant correlation between PPARγ2 gene Pro12Ala polymorphism and Ningxia Hui and Han T2DM poputlation,; 2,PPARγ2 gene Pro12Ala polymorphism (PA/AA) genotype may be associated with obesity; 3,Age, family history of diabetes, high Blood pressure, BMI, WHR, LDL is a risk factor for T2DM, HDL areprotective factors of T2DM.