The Therapeutic Effects Of M4 To Ovarian Cancer Cell Lines And The Primary Mechanism

Objective: To investigate the therapeutic effect of M4 in the ovarian cancer cell lines OV2008 and C13K in vitro, meanwhile to investigate the primary mechanism of M4, which can reverse the drug resistance of C13K to DDP.Methods: After the infection of M4 to the cells mentioned above, the inhibition rate of proliferation can be measured by MTT; the rate of apoptosis was measured by flow cytometry; We carried out the Western Blot to evaluate the protein expression of STAT3 and its target protein; Transwell can elluminate the invasive ability of the cells. DDP was used to ovarian cancer cell C13K, and then the inhibition rate of proliferation can be tested by MTT, also the rate of apoptosis can be measured by flow cytometry. Both M4 and DDP were added to C13K, and then the inhibition rate of proliferation can be tested by MTT, besides Real-time PCR was applied to evaluate the expression of MDR-1.Result: After the infection of M4, the proliferation of OV2008 and C13K was inhibited obviously (p<0.05), the apoptotic rate of these cell lines was obviously raised (p<0.05); Western Blot showed that after the infection, the protein expression level of STAT3 and its target protein: MMP-9, Survivin, Bcl-xl, Cyclin D1 were all decreased (p<0.05); Transwell showed the invasive ability of ovarian cancer cell was decreased. DDP has little effect to the rate of proliferation and apoptosis of the ovarian cell line C13K. But when combined with M4, the rate of proliferation was decreaed obviously and MDR-1 was decreased, too.Conclusion: M4 can be effective to ovarian cancer cells OV2008 and C13K. And the primary mechanism may be attributed to its blockage to the expression of STAT3, which triggered the decreased expression of p-STAT3 and its target protein MMP-9, Bcl-xl, Survivin, Cyclin D1 et al, and ultimately resulted in the apoptosis of cancer cells. M4 combined with DDP can decrease expression of MDR-1 and the rate of proliferation in C13K.