| Objective The aim of this study was to research the influence of aspirin-triggered lipoxin on inflammatory response induced by lipopolysaccharide in rat primary astrocytes.Methods Research model was established using brain cortices cell from 1- to 3-day-old Sprague-Dawley rats.Purified primary astrocytes were divided randomly into control group,LPS group, ATL+LPS groups and ATL group, The expressed quantities of NO and PGE2 in the supernatants of the cultured cells were detected by nitrate reductase method and ELISA.The expression of iNOS and COX-2 mRNA were assayed by RT-PCR, and the expression of iNOS and COX-2 protein were detected by Western blotting.Results (1)ATL could reduce the thoughput of No and PGE2. Compared with control group ,The ATL group had unconspicuous influence on the quantities of NO and PGE2 in the supernatants of the cultured cells. There were no invident differences in the level of No and PGE2 between LPS group and low dose of ATL+LPS group. But high dose groups (10nm/mL and 100nm/mL),brought down the quantities of NO and PGE2. (2)ATL could inhibit the expression of iNOS and COX-2.Compared with LPS group.The ATL (10nm/mL and 100nm/mL)+LPS groups decreased the concentration of the mRNA level of COX-2 and iNOS. Also,the high dose group did an significantly decrease.In the protein level, the expression of iNOS and COX-2 protein were attenuated in ATL(100nm/mL)+LPS group comparing with LPS group .Conclusions These results demonstrated that ATL could keep down the inflammatory response in rat primary astrocytes.It was showed that ATL could decrease the thoughput of No and PGE2,keep down the expressin of iNOS and COX-2 induced by LPS. |
