A Preliminary Study On Determining The Optimal Time Point For Transplantation Of Neural Stem Cells

ObjectiveTo explore whether the neural stem cells (NSCs) could survive in artificial cerebrospinal fluid (ACSF).MethodsAfter in vitro expansion for two passages in standard media, NSCs were respectively seeded and cultured in the ACSF or normal neural media. The characteristics of NSCs growing in the two media were observed and compared. Immunofluorescence staining was adopted to detect the expression of Tubulin and GFAP of cells in two media at the third day.ResultsThe NSCs survived and grew in ACFS. The immunofluorescence staining showed Tubulin(+) and GFAP(+). But NSCs in ACFS had a slender body and synaptic compared with those in ordinary neural media.ConclusionIn this experiment condition, the NSCs could survive, grow and differentiate in ACFS. ObjectiveTo explore the optimal time point for neural stem cells (NSCs) to transplant in artificial cerebrospinal fluid (ASCF) in vitro.MethodsAfter in vitro expansion for two passages in standard media, NSCs were induced to differentiate, and then they were harvested respectively from day 1 to day 6 in the induced differentiation. Cells were seeded in the ASCF and cultured for another 3 days. The positive rate of neuron from the NSCs was calculated. MTT was adopted to explore the relative growth activity of the cells.ResultsIn this experiment condition NSCs survived and grew in the ASCF. Statistical analysis showed cells differentiated for 4 days got a significantly higher positive rate of neuron and relative growth activity in ASCF than other time points (P<0.05).ConclusionIn this experiment condition differentiation for 4 days was the optimal time point for NSCs to transplant in ASCF in vitro...