| Objective:To develope a rat model of Intracerebral Hemorrhage (ICH), evaluate the neural function of rats, and detect the histopathological changes around the hematoma at different time points after ICH.Methods:Forty adult SD rats were randomly divided into control group and ICH group.CollagenaseⅦ/normal saline solution was stereotactically injected into the right basal ganglia of rats in ICH/control group. The neural function of rats was evaluated with Zea Longa 5-grade scale and the histopathological changes around the hematoma were detected by HE staining at 1, 3, 7, 14 and 28 days after ICH.Results:A rat model of ICH was successfully developed. There was no difference of scores of neural function evaluation before and after surgery of rats in control group. The scores of neural function evaluation of rats in ICH group increased gradually and reach the peak 3 days after collagenase injection. The scores at 3 d was significantly higher than those at 14 d and 28 d after collagenase injection (P<0.05). An apparent hematoma was observed at rat basal ganglia area 1 d after collagenase injection. Brain edema increased and intercellular space broadened 3 days after collagenase injection. Astrocytosis was observed 7 days after collagenase injection. Irregular capsular space of hemorrhagic focus began to form 14 days after collagenase injection. Astrocytosis was more serious and capsular space still remained 28 days after collagenase injection.Conclusion:Rat ICH model produced with induction by collagenase typeⅦ, with low mortality, high success rate and stable volume of hematoma, is an ideal model for experimental ICH. The features of neural function and histopathologcal changes help to investigate the therapeutic time window of ICH. Objective: To observe the migration, differentiation and function of adipose-derived stem cells (ADSCs) after stereotaxic transplanting into lateral cerebral ventricle of rats with intracerebral hemorrhage (ICH).Methods: ADSCs were cultured, proliferated, identified and conjugated with BrdU in vitro. Fifty SD rats with ICH were randomly divided into ADSCs group and control group.ADSCs/normal saline solution was stereotactically injected into the right lateral cerebral ventricle of rats in ADSCs/control group 48 hours after ICH. Zea Longa 5-grade scale was adopted to evaluate the neural function of rats, fluorescent immunostaining staining was adopted to detect the expression of NeuN and GFAP of ADSCs, TUNEL staining was adopted to observe the apoptosis of cells, and immunohistochemical staining was adopted to detect the expression of vascular endothelial growth factor (VEGF) at 1, 3, 7, 14 and 28 days after ICH.Results: ADSCs showed osteogenic and adipogenic differentiation ability in vitro. Compared with those in control group, rats in ADSCs group showed better motor function at 3, 7, and 14 days after transplantation (P<0.05). Double immunofluorescence staining showed most of the BrdU-conjugated ADSCs migrated to the hematoma zone and some expressed NeuN or GFAP. TUNEL staining showed that apoptotic cells in rats of ADSCs group were significantly less than that in control group at 3 days after transplantation (P<0.05). VEGF expression in rats of ADSCs group was significantly higher than that in control group at 3 days after transplantation (P<0.05).Conclusion: ADSCs transplanted into the lateral ventricle can survive and differentiate into neuron-like cells. Transplantation of ADSCs helps to reduce cell apoptosis, promote VEGF secretion and improve neurological function of rats with ICH. |
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