Bone Marrow Microenvironment Mediates Aml Cells Drug Resistance And Mechanism Reseach

Objective: To simulate AML bone marrow microenvironment, it researches AML cell HL-60 chemoresistance change to daunorubicin after exposure to bone marrow stromal cells MSC, and discusses part mechanism that MSC mediated the HL-60 drug resistance.Measures: Establishing co-cultivating drug-resistant model by HL–60 cell and MSC of patient with AML in vitro; Using flow cytometry for detect effect of apoptosis by MSC and FN on chemotherapy HL-60 cell; To detect FN in MSC condition medium and co-culture medium by Elisa; Usin CCK8 for detect effect of inhibition rate by MSC condition medium and FN on chemotherapy HL-60 cell; Using flow cytometry for analyze cell cycle of cultivated HL-60 with MSC; Western Blot discusses mechanism that MSC and FN mediate HL-60 drug resistance.Resμlts: Co-culture of MSC and HL-60 can obviously reduce cell apoptosis of chemotherapy HL-60. MSC can secrete much FN that mediates HL-60 cell drug resistance. MSC mediates HL-60 cell drug resistance may be due to activate ILK,P-AKTprotein and down-regulate P-P38 in HL-60 cell and FN participates this process.Conclusion: Bone marrow stroma cells mediated HL-60 Chemoresistance to daunorubicin and FN participated this process.