Effect Of SiRNA Targeting CXCR4 On The Proliferation And Invasion Of Ovarian Cancer Cells

ObjectiveAccording to the RNA interference can specifically block the expression of targeted genes,we design and construct small interfering RNA plasmid of chemokine receptor 4. The siRNA of CXCR4 plasmid is tansfected into SW626 cells, and to investigate the inhibitory effect of CXCR4 siRNA on the proliferation, invasion and metastasis capability of ovarian cancer cell SW626 cells in vitro.MethodsConstruction, amplification and identification the siRNA of CXCR4 plasmid; the siRNA of CXCR4 plasmid was transfected into SW626 cells by lipofectamineTM2000; the silence rates of CXCR4 mRNA and protein expression were detected by real time reverse transcription-polymerase chain reaction and Western Blot respectivly; the cell proliferation capability was investigated by MTT assay; the cell invasion and metastasis capability in virto was estimated by Transwell experiment.ResultsWe successfully constructed the siRNA of CXCR4 plasmid with three specific fragment. The transfection efficiency of ovarian cancer cell line SW626 cells was approximately 80%-90% after transfected 48 hours. In contrasted to control group, the expression of CXCR4 mRNA and protein was significantly decreased in interference group (P <0.01). And the cell viability was also partially inhibited which had no relationship to time. Cell migration and invasion abilities were reduced significantly in interference group (P <0.01).ConclusionsThe siRNA targeted for CXCR4 can efficiently inhibit the expression of CXCR4 mRNA and protein and decrease proliferation, invasion and metastasis capability of SW626 cells. Our work will provide the theoretical basis for genes interference therapy of ovarian cancer.