| Objective:To study the functions and correlations during the development and progress of primary epithelial ovarian cancer by detecting the expressions of PI3K and AKT of PI3K/AKT signaling pathway genes, Survivin and XIAP of Inhibitor of apoptosis proteins (IAPs) members,and Cyt-c,Caspase-9,Bax and Bcl-2 of regulation of the mitochondrial apoptosis pathway-related factors in normal epithelial ovarian tissues, ovarian benign tumor, borderline ovarian tumors and primary epithelial ovarian cancer.Methods:Expressions of PI3K,AKT,Survivin,XIAP,Cyt-c,Caspase-9,Bax andBcl-2 were detected by IHC and Image-ProPlus image analysis software in in 15 cases of normal epithelial ovarian tissues, ovarian benign tumor, borderline ovarian tumors and 30 cases of primary epithelial ovarian cancer.Results:1,The expression of PI3K, AKT, Survivin, XIAP, Cyt-c, Caspase-9, Bax and Bcl-2 in normal ovarian tissues (normal group), ovarian benign tumors (benign group), borderline ovarian tumor (borderline group) and primary epithelial ovarian cancer (malignant group):(1)The positive expression rates of PI3K of PI3K/AKT signaling pathway in normal, benign, borderline and malignant groups were respectively 13.3%,13.3%,20.0%,56.7%. The differences were statistically significant (P<0.05)during groups which malignant group was respectively compared with normal,benign and borderline groups. The differences were not statistically significants (p>0.05)during groups which borderline group was respectively compared with normal and benign groups, and benign group was compared with normal group.The positive expression rates of AKT in normal, benign, borderline and malignant groups were respectively 13.3%,26.7%,33.3%,56.7%.The differences were statistically significant (P<0.05)during groups which malignant group was respectively compared with normal,benign and borderline groups. The differences were not statistically significants (P>0.05)during groups which borderline group was respectively compared with normal and benign groups, and benign group was compared with normal group.(2)The positive expression rates of Survivin of inhibitor of apoptosis protein family member in normal, benign, borderline and malignant groups were respectively 0%,13.7%, 26.7%,76.7%. The differences were statistically significant (P<0.05)during groups which malignant group was respectively compared with normal,benign and borderline groups, and benign group was compared with normal group.The differences were not statistically significants (P>0.05)during groups which borderline group was respectively compared with normal and benign groups.The positive expression rates of XIAP in normal, benign, borderline and malignant groups were respectively 0%,13.3%,20.0%,73.3%. The differences were statistically significant (P<0.05)during groups which malignant group was respectively compared with normal,benign and borderline groups. The differences were not statistically significants (P>0.05)during groups which borderline group was respectively compared with normal and benign groups, and benign group was compared with normal group.(3)The positive expression rates of Cyt-c of mitochondrial pathway of apoptosis regulatory factors in normal, benign, borderline and malignant groups were respectively 40%,46.7%, 60%,93.3%. The differences were statistically significant (P<0.05)during groups which malignant group was respectively compared with normal,benign and borderline groups. The differences were not statistically significants (p>0.05)during groups which borderline group was respectively compared with normal and benign groups, and benign group was compared with normal group.The positive expression rates of Caspase-9 in normal, benign, borderline and malignant groups were respectively 13.3%,20%,33.3%,66.7%. The differences were statistically significant (P<0.05)during groups which malignant group was respectively compared with normal,benign and borderline groups. The differences were not statistically significants (P>0.05)during groups which borderline group was respectively compared with normal and benign groups, and benign group was compared with normal group. The positive expression rates of Bax in normal, benign, borderline and malignant groups were respectively 13.3%,13.3%,20%,53.3%. The differences were statistically significant (P<0.05)during groups which malignant group was respectively compared with normal,benign and borderline groups. The differences were not statistically significants (P>0.05)during groups which borderline group was respectively compared with normal and benign groups, and benign group was compared with normal group.The positive expression rates of Bcl-2 in normal, benign, borderline and malignant groups were respectively 13.3%,13.3%,26.7%,60%. The differences were statistically significant (P<0.05)during groups which malignant group was respectively compared with normal,benign and borderline groups. The differences were not statistically significants (P>0.05)during groups which borderline group was respectively compared with normal and benign groups, and benign group was compared with normal group.2.The relationship between expression intensities(value of MOD) of detection targets in epithelial ovarian cancer and clinical pathological features:The levels of protein expressions (value of MOD) of PI3K and AKT in advanced clinical stage (stage and stage ) group were significantly higher than that of the early clinical stage (stage and stage ) group (P<0.05), and were not related with patient age, pathological type and lymph node metastasis (P>0.05).The levels of protein expressions (value of MOD) of Survivin and XIAP were significantly higher than that of the early clinical stage (stage and stage ) group (P<0.05), and were not related with patient age, pathological type and lymph node metastasis (P>0.05) in advanced clinical stage (stage and stage ) group.The level of protein expressions (value of MOD) of Cyt-c and Bcl-2 were significantly higher than that of the early clinical stage (stageⅠand stageⅡ) group (P<0.05) in advanced clinical stage (stage and stage ) group. The level of protein expression (value of MOD) Caspase-9, Bax in the late clinical stage (stageⅠand stageⅡ) group, were significantly higher than that of the early clinical stage (stageⅢand stageⅣ) group (P<0.05). The protein expression of Cyt-c was related with lymph node metastasis (P<0.05), and was not related with age and pathological types (P>0.05). The protein expressions of Caspase-9, Bcl-2 and Bax were not related with patient age, pathological type and lymph node metastasis (P>0.05).3.The correlation analysises of PI3K, AKT, Survivin, XIAP, Bcl-2, Bax in epithelial ovarian cancer:PI3K and AKT was positively correlated (r=0.464, P=0.010<0.01), PI3K and Survivin was positively correlated (r=0.411, P=0.024<0.01), AKT and Survivin was positively correlated (r=0.369,P=0.045<0.01), Survivin and Bcl-2 was positively correlated (r=0.471, P=0.009<0.01), Bax and Bcl-2 was negatively correlated(r=-0.386, P=0.035<0.01). PI3K,AKTwith Survivin had no correlations, Survivin and XIAP had no correlation.Conclusion:1,The expressions of PI3K, AKT, Survivin, XIAP, Cyt-c and Bcl-2 in epithelial ovarian carcinoma obviously increase, suggesting that they have close relationship with the incidence of epithelial ovarian cancer.Their high expressions can be used as references of diagnostics in epithelial ovarian cancer.2, The expressions of PI3K, AKT, Survivin, XIAP, Cyt-c and Bcl-2 are related with clinical stage.Expressions of these factors indicate poor prognosises.3, The expression of Survivin can promote high expressions of PI3K, AKT and Bcl-2 in epithelial ovarian cancer.4, PI3K,AKT,Survivin,XIAP,Caspase-9,Bax and Bcl-2 are not related with patient age, pathological type and lymph node metastasis. |
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