The Reseach Of The Action Of Cinnamaldehyde Solution On Tumor Necrosis Factor-α Producting By Human Periodontal Ligament Fibroblast Cells Stimulated By The LTA From Actinomyces Naeslundii

Periodontitis is a common and chronic inflammatory oral disease caused by bacteria leading to destruction of tissues supporting the teeth. Besides that, the toxins produced by these bacteria and their antigens, inducing the periodontal tissue cells to produce cytokines, increases periodontal Inflammation.as periodontitis's relation pathogens, actinomyces naeslundii can induce a large amount of tumor necrosis factor (TNF-α), to lead to cell necrosis collapse and direct destruction of periodontal tissue. Extracted from cinnamon, cinnamaldehyde was found to inhibit some bacteria growing, and IL-1β, TNF-αproduction from cells. It is not clear.whether it can inhibit the production of TNF-αfrom periodontal ligament fibroblast cells induced by actinomyces naeslundii lipoteichoic acid (LTA).ObjectiveThe study was done to observe that the cinnamaldehyde can inhibition the growth of actinomyces naeslundii and to measure the effect from cinnamaldehyde on the production of TNF-αfrom periodontal ligament fibroblast cells stimulated by the LTA from actinomyces naeslundii. Finally, to find the theory basis of the cinnamaldehyde to treat the periodantitis.MethodsIn vitro antibacterial test:The density of atinomycets naeslundii strains ATCC12104 was 5×103 cells/ml. The solution of metronidazole and cinnamaldehyde were diluted into 1024,512,256,128,64,32μg/ml; metronidazole as the control group. The cinnamaldehyde and metronidazole with different concentrations was put into actinomyces naeslundii strains in the TSB liquid media cultured carbon dioxide for 24 hours. The growth of bacteria on the smears were observed with a microscope.Inhibition of production of TNF-α: The periodontal ligament fibroblast cells were cultured in vitro and used fourth generation in the experiment. The wet bacteria was centrifugated and added an equal volume of butanol to extract the cell wall components as the crude extraction. The crude extraction as the frozen white powder was dissolved in chromatography buffer and purified by CL-4B agarose gel chromatography (LTA). Then periodontal ligament fibroblast cells (density of 5×103 cells/ml) were cultured with LTA as the different concentrations of 130.9,65.45,32.72,16.36,8.18 mg/ml, and ELISA was used to detect TNF-a in the cell culture supernatant. The cinnamaldehyde was preparated as concentration of 256,128,64,32,16μg/ml and put into the culture of periodontal ligament fibroblast cells with the LTA for 24 hours, ELISA was used to detect TNF-a in the cell culture supernatant.Results1. Cinnamaldehyde significantly inhibits the growth of actinomyces naeslundii, therefore, resulting in the minimum inhibitory concentration of MIC being 64μg/ml.2. LTA can induce periodontal ligament fibroblast cells to produce TNF-a, the LTA generated when the maximum concentration of 16.36mg/ml.3. Cinnamaldehyde significantly inhibited the TNF-a producde from periodontal ligament fibroblast cells stimulated by LTA.With increasing concentrations of cinnamaldehyde, the stronger the inhibition.Conclusion1. The actinomyces naeslundii's wall lipoteichoic acid (LTA) could induce production of TNF-a by stimulating the periodontal ligament fibroblasts.2. cinnamaldehyde can inhibite the TNF-αproduced from periodontal ligament fibroblast cells induced by the LTA from actinomyces naeslundii.