Expression Of Annexin A7 In Laryngeal Squamous Cell Carcinoma

Objective: To study that the expression of Annexin A7 in laryngeal squamous cell carcinoma (LSCC) is different from that in adjacent normal laryngeal mucosa and make it certain that Annexin A7 may play an important role in the oncogenesis and progression of LSCC as a tumor suppressor gene and provide a kind of clinical target for observing and assessing the malignant potential of laryngeal mucosa.Methods: The experiment detects the protein expression of Annexin A7 by immunohistochemistry (MaxVisionTM) in 31 cases of LSCC and 32 cases of adjacent normal laryngeal mucosa tissues and analyze the relationship between the expression of Annexin A7 and age, gender, anatomical district, pathological grade or clinical stage of the laryngeal cancer patients.Results:1 Annexin A7 protein is abundant in the normal laryngeal mucosa and is found localized in the cell membrane by immunohistochemistry. It is manifested a brown granular or line network distribution.2 The expression of Annexin A7 can be divided into four grades. They are negative, weakly positive, positive and strongly positive respectively. In the 31 cases of LSCC, There are 23 cases negative, 5 cases weakly positive, 1 case positive and 2 cases strongly positive. However, in the 32 cases of adjacent normal laryngeal mucosa tissues, there are 1 case negative, 2 cases weakly positive, 9 cases positive and 20 cases strongly positive. There is much difference between the two groups which is tested statistically significant by means of nonparametric tests. The expression of Annexin A7 in LSCC is much lower than that in the adjacent normal laryngeal mucosa.3 All the 31 cases of LSCC patients can be divided into two groups according to the anatomical district, pathological grade or clinical stage. The expression of Annexin A7 in two groups is tested by means of nonparametric tests and it is found that there is no significant difference. By the same means, the relationships among the expression of Annexin A7 and the age, gender, anatomical district, pathological grade or clinical stage of the laryngeal cancer patients are tested to be no significant difference.Conclusion:1 The Annexin A7 protein is widespread in cells. It is localized in the cell membrane in the laryngeal epithelial cells.2 The expression of Annexin A7 in the LSCC compared with that in the adjacent normal laryngeal mucosa is much decreased. The expression loss in the LSCC may play an important role for early diagnosis of LSCC and for predicting the cancer risk of the normal laryngeal mucosa.3 The expression of Annexin A7 has no relation with age, gender, anatomical district, pathological grade or clinical stage of the laryngeal cancer patients.