| Objective:To investigate the serum homocysteine (Hey), endothelial microparticles (EMPs) in preeclampsia, compare the variation prepartum and postpartum.Methods:27 women of preeclampsia (severe preeclampsia 17 cases, mild preeclampsia 10 cases) and 19 normal term pregnant women were collected. Their peripheral blood was respectively collected within 24h after their admission and the third day after Cesarean section. The serum Hey was detected by liquid chromatographic-tandem mass spectrometry (MS) detection (LC-MS/MS). And the levels of corresponding serum EMPs were detected by flow cytometric analysis. The relevance of concentrations of Hey and EMPs with mean arterial pressure (MAP) and urine protein were analysed.Results:1. The serum Hey in the preeclampsia group was significantly higher than that of the normal term pregnant group (10.97±3.32 umol/L.vs.7.28±2.17 umol/L, P<0.01), The serum Hey of the severe preeclampsia group was significantly higher than that of the mild preeclampsia group (12.10±2.91 umol/L.vs.8.82±3.08 umol/L, P<0.01), The serum Hey of the mild preeclampsia group had no significant difference compared with that of normal term pregnant group (P>0.05). The postpartum serum Hey of the preeclampsia group was significantly higher than that of the normal term pregnant group (10.17±2.40 umol/L.vs. 7.68±2.08 umol/L, P<0.01). The postpartum serum Hey of the severe preeclampsia group was significantly higher than that of the mild preeclampsia group (10.90±2.47 umolL.vs. 8.79±1.58 umol/L, P<0.05). The postpartum serum Hcy of the mild preeclampsia group had no significant difference compared with that of normal term pregnant group (P>0.05). In every group, the prepartum serum Hcy had no significant difference compared with the postpartum serum level (P>0.05). 2. The serum EMPs of the preeclampsia group was significantly higher than that of the normal term pregnant group (2863.5±1543.1/ml.vs.1703.0±592.2, P<0.01). The serum EMPs of the severe preeclampsia group was significantly higher than that of the mild preeclampsia group (3467.5±1440.1/ml.vs.1836.7±1153.8/ml, P<0.01). The serum EMPs of the mild preeclampsia group had no significant difference compared with that of normal term pregnant group (P>0.05). The postpartum serum EMPs had no significant difference in every group. The prepartum serum EMPs of was significantly higher than the postpartum serum level in the preeclampsia group and the severe preeclampsia group (2863.5±1543.1/ml.vs.1262.0±685.8/ml, P<0.01; 3467.5±1440.1/ml.vs.1421.8±760.2/ml, P<0.01). The prepartum serum EMPs had no significant difference compared with the postpartum serum level in the mild preeclampsia group and normal term pregnant group (P>0.05).3. There was significantly positive correlation and linear regression between serum Hcy and the MAP as well as 24h urine protein of preeclampsia group(R=0.53, P<0.01; R=0.68, P<0.01). similarly, there was significantly positive correlation and linear regression between the level of EMPs in serum and the MAP as well as 24h urine protein of preeclampsia group(R=0.33, P<0.05; R=0.67, P<0.01).4. There was significantly positive correlation and linear regression between the level of Hcy and the EMPs in serum(R=0.59, P<0.01).Conclusion:1. The serum Hcy and EMPs in preeclampsia group was significantly higher than that of the normal term pregnant group. The serum Hcy and EMPs in the severe preeclampsia group was significantly higher than that of the mild preeclampsia group. They had positive correlation and linear regression with the MAP and 24h urine protein. From all of this, it suggests that Hcy and EMPs may be play important role in the pathogenesis of preeclampsia.2. In each group, the postpartum serum EMPs was significantly descended compared with that before termination in the preeclampsia group. It suggested termination of pregnancy can modify endothelial function, and it provided direct evidences to cordinated the theory of endothelial injury in the pathogenesis of preeclampsia. |
PDF Full Text Request