Experimental Biocompatibility And Biomechanical Evaluation Of Silk Protein-coated Polypropylene Meshes

Part 1 : The Biocompatibility of silk protein–coated polypropylene in an abdominal hernia rabbit model.Objective: The purpose of this study was to determine whether the host tissue response in the abdominal hernia rabbit model differs for 2 polypropylene meshes(coated and noncoated)Methods:Twenty-four 30×30mm polypropylene (12 Gynemesh?? and 12 Aspide?) meshes (6 coated and 6 noncoated) were surgically implanted in 6 abdominal hernia rabbit model.which had Six abdominal wall defects for each. Four abdominal wall defects for Coated/noncoated Gynemesh? and Surgmesh?,two left as the control. Animas were killed 30 (n=2),60 (n=2) and 90 (n=2) days after surgery.Full-thickness sections of abdominal wall were evaluation included macroscopical examination,histological and immunohistochemical analysis.,for example,inflammation,neovascularization, fibroblastic proliferation and collagen formation. Results:Erosion of meshes did not occur in any animal. silk protein–coated low-weight polypropylene meshes induced a mild inflammatory response with minimal fibrosis and good host tissue incorporation within the grafts.Few apoptotic and necrotizing cell were found in noncoated meshes.Conclusion: coated meshes elicit a mild foreign body reaction ,minimal fibrotic response and good host tissue incorporation within the graftsPart 2:Biomechanical properties of silk protein–coated polypropylene in an abdominal hernia rabbit model.Objective: To study the effects of silk protein coating on the polypropylene meshes biomechanics.Methods: 3.5cm×3.5cm full-thickness tissues of the New Zealand rabbits abdominal wall for 30 days, 60 days, 90 days postimplantation.were divided into three strips ,3.5cm×1cm for each .Took the middle one for tensile test, statistical analysis for the maximum tensile stress of broken, deformation of broken, tensile stress for fail and Young's elastic modulus.Results: Tension parameter of all meshes were higher than the control group Tensile stress for broken of noncoated meshes lowter than coated meshes of which coated Gynemesh ? was the highest Tension properties after implantation was better than before, Biomechanical properties Improved over time.Conclusion: Silk protein coating can improve the biomechanical properties. of polypropylene meshes...