| Objectives:Study on leptin receptor (Lepr) gene Lysl09ArgA/G, Ala976AspC/A polymorphism with obesity and obstructive sleep apnea hypopnea syndrome(obstructive sleep apnea-hypopnea syndrome,OSAHS) relevance. Serum leptin levels were also discussed and the relationship between OSAHS and obesity. Simple to find and place OSAHS and OSAHS with obesity-related genotypes, genetic markers and the search for possible predisposing factors for OSAHS susceptible population screening, prevention and diagnosis to provide a new target.Method:Select from Februery 2010to December 2010 in North China Coal Medical College hospital Kailuan, the existence of clinical investigations at night snoring, apnea and daytime sleepiness medical history, and by polysomnography (polysomnography, PSG) monitoring confirmed 184 patients with obstructive sleep apnea syndrome patients as research subjects, of which 90 patients with OSAHS alone (alone OSAHS group), aged 35 ~ 63 (42.4±2.5) years of age, body mass index (22.60±1.53) kg/m~2; 93 cases of obese patients with OSAHS (OSAHS combined obese group), aged 32 ~ 61 (41.4±3.5) years of age, body mass index (28.80±1.74) kg/m~2. Select the same group identified by the hospital medical centers shape the health and normal (control group) 91 patients aged 34 ~ 65 (43.4±3.5) years of age, body mass index (22.11±1.68) kg/m~2; other selected healthy people, But the body obese (obesity group) 94 patients aged 33 ~ 64 (42.4±2.5) years of age, body mass index (28.91±1.56) kg/m~2. OSAHS diagnosis of respiratory diseases according to the Chinese Medical Association credits will sleep study group developed respiratory disease diagnostic criteria: the sleep apnea-hypopnea index (AHI, sleep apnea per hour combined with low ventilation frequency)≥5 times / h, and mainly to obstructive apnea, the diagnosis of OSAHS. Body mass index (BMI)≥25 diagnosed as obese. Comparison among the four groups the number, age was no significant difference (p> 0.05), body mass index (BMI) compared OSAHS combined with obesity group and the obese group was no significant difference, the simple OSAHS group and the healthy control group, no significant differences (all p> 0.05); OSAHS combined with the simple obesity group OSAHS group and the obese group compared with the healthy control group were significantly different (p <0.05). All subjects filled out questionnaires and physical examination records of all investigations. All subjects were monitored at the end of sleep apnea, morning wake up 5 minutes fasting venous blood samples (4ml), for the determination of fasting blood glucose (FBG) and other biochemical indicators. In another EDTA-tubes, venous blood 3ml, white blood cells by hypotonic extraction, phenol - chloroform extraction of DNA, polymerase chain reaction - restriction endonuclease (PCR-RFLP) were detected Lepr gene Lysl09ArgA/G, Ala976AspC/A polymorphis.By enzyme-linked immunosorbent assay (ELISA) serum leptin levels. Build a database of all research data, using SPSS13.0 statistical package for analysis. Statistically significant difference in taking the level of P <0.05. Results:Leptin receptor (Lepr) gene Lysl09ArgA / G locus and allele frequency distribution as follows: AA, AG, GG genotypes were all as simple OSAHS5 cases (5.5%), 14 patients (15.5 %), 71 cases (79.0%); OSAHS combined with obesity group 4 cases (4.3%) in 12 cases (12.9%) 77 cases (82.7%) healthy control group 3 patients (3.2%), 14 patients (15.3%), 74 cases (81.3%); simple obesity group 2 patients (2.1%), 15 patients (15.9%), 77 patients (81.9%); carry A and G allele frequencies were: 21.1% pure OSAHS group, 94.4%; OSAHS combined obese group 17.2%, 95.7%; healthy control group 18.7%, 96.7%, 18.1% obese group, 97.8%; Between the two groups was no significant difference (P> 0.05). Leptin receptor (Lepr) gene Ala976AspC/A site mutation frequency of 100%, polymorphism showed CC, AA two in each group CC, AA genotypes were: simple OSAHS group of 11 patients (12.2%) , 79 cases (87.8%); OSAHS combined with obesity 9 cases (9.7%), 84 patients (90.3%); healthy control group of 10 patients (10.9%), 81 patients (89.1%) obese group of 10 patients (10.6%) , 84 (89.4%). A and G allele carrying genotype frequency and the same frequency. No significant difference between the two groups (P> 0.05). Leptin receptor gene Lys109Arg GG-type phenotype in the hip was 108.34±4.50cm, AG + AA-type hip 102.12±3.23, compared two groups of type GG> AG + AA-type, the difference was statistically significant (p <0.05 .) Serum leptin levels in four groups: OSAHS combined obese group (17.85±7.86) ng / ml OSAHS group than alone (13.42±6.11) ng / ml higher, statistically significant difference between the groups (p <0.05); OSAHS and obesity and serum leptin alone OSAHS group than in the obesity group (12.25±4.72) ng / L, the control group (7.86±2.02) ng / L higher, statistically significant difference between the groups (p <0.05). Simple serum leptin levels in OSAHS patients than in healthy control group increased (P <0.05). OSAHS patient group than the control group TG, LDL-c levels, were significantly different (P <0.05).With the increase of obesity, neck circumference of the thickening, OSAHS level increased. Methods of Multivariate Logistic susceptibility to obesity factors and association between OSAHS screening age (40 to 60 years), smoking history, family history of snoring, neck circumference of four risk factors, with statistical significance of these factors may increased risk of OSAHS.Conclusion:1. Leptin receptor Lysl 0 9 ArgA/G, Ala976AspC/A gene polymorphism and incidence of OSAHS has nothing to do, had no effect on serum leptin expression. 2. Leptin receptor (Lepr) Lysl 0 9 Arg gene polymorphism and obesity increase the hip may be relevant.3. Comparison of serum leptin levels, OSAHS combined with obesity were significantly higher than that OSAHS group, body fat was significantly higher than the normal control group, the control group, the differences were statistically significant, while the obesity group compared with the simple OSAHS group, no significant difference significance. |
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