| Objective:The subject of sports through the establishment of chronic fatigue model and general observation, mouse tail suspension test, exhaustive swimming test, observe the behavior Bugan decoction on peripheral blood lymphocyte apoptosis, liver function, liver, gastrocnemius morphology study, liver, gastrocnemius muscle SOD activity, MDA content, Bugan soup in the treatment of chronic exercise-induced fatigue and its mechanism, and thus chronic exercise-induced fatigue associated with liver stores like.Methods:Healthy male SD rats were randomly divided into 3 groups: normal group, model group, treatment 1 (Bugan soup),2 (CHSGS), 3 (Four Gentlemen Decoction),4 (Pill) group 10 in each group. After the administration to observe the rat model of chronic exercise-induced fatigue behavior, peripheral blood lymphocytes, liver function, liver and gastrocnemius muscle tissue morphology, liver, gastrocnemius muscle SOD activity, MDA content changes. ResultsResults1. Chronic Fatigue behavioral changes in ratsTail suspension test results:group 1 treated rats the number of fixed time and struggling to change and no significant difference between the normal group (p<0.01); with the model group was significantly shorter immobility time, while struggling to increase the number of (p<0.01), treatment group 1 the most significant (P<0.05); compared with the treatment of 2,3 immobility time in rats with different degrees of shortening, struggling to increase the number (p<0.05); treatment of a fixed time and the rats struggle to change the number and treatment no significant difference between group 4 (P<0.01).Exhaustive swimming test:the treatment 1 group compared with the normal group exhaustive time no significant difference (p<0.01); treatment of a rat model of exhaustion was longer than group (p<0.05), exhaustive treatment of a group significantly shorter in the treatment group 1 (p<0.05); treatment 1 and treatment group exhaustive time no significant difference between group 4 (P<0.01).2. Chronic Fatigue rat peripheral blood lymphocytes apoptosis rateFatigue syndrome treatment group 1 on the apoptosis rate of peripheral blood lymphocytes of small difference between the normal group (p<0.01); fatigue syndrome treatment group 1 on the apoptosis rate of peripheral blood lymphocytes of low in the untreated group (p<0.05), treatment group 1 on the fatigue syndrome in peripheral blood lymphocyte apoptosis rate of less than 2,3-treatment group (p<0.05); treatment group 1 rats on peripheral fatigue syndrome Influence of blood lymphocyte apoptosis and treatment is no big difference between 4 groups (P<0.01).3. Liver function changesExperimental treatment group 1 serum AST, ALT Content smaller difference compared with the normal (p<0.01); treatment group 1 on rat serum AST, ALT content was significantly lower than model group (p<0.05), treatment group 1 on rat serum AST, ALT levels of less than 2,3-treatment group (p<0.05); treatment group 1 on rat serum AST, ALT levels of treatment for 4 groups There was no big difference (P<0.01).4. Chronic Fatigue liver, morphological changes of gastrocnemiusChronic exercise-induced fatigue in rats gastrocnemius muscle tissue optical microscopy results are as follows: gastrocnemius cross-section of skeletal muscle cells can be seen disorder, part of the muscle fiber membrane swelling and dense nuclei, the number increased, a small number of muscle fiber hypertrophy, with vacuolar degeneration, or a muscle fiber is not continuous, visible lipofuscin deposition. Rat model of chronic fatigue can be seen in the gastrocnemius muscle movement organizations have taken place in disease, the treatment group were seen gastrocnemius cross-section of skeletal muscle cells have a different degree of improvement, the most obvious Bugan decoction group compared with the normal group is not much difference. Similarly, chronic exercise-induced fatigue model of rat liver tissue in the optical microscope:the destruction of hepatic lobules, liver cell disorder, irregular, showing cell clusters and clear cell proliferation, increased liver nuclei and nucleoli increased, showing that cytoplasmic vacuoles, a de-nuclear phenomena. Description of Chronic Fatigue on the influential rat liver cells, rat liver tissue has been destroyed, treated rats improved the symptoms of liver cell damage, the most obvious Bugan decoction group compared with the normal group difference is not too large.5. Chronic Fatigue liver, gastrocnemius muscle SOD activity, MDA content inTreatment group 1 in rat liver tissue decreased SOD activity, MDA content increased with no significant difference between the normal group (p<0.01); treatment group 1 in rat liver tissue SOD activity was significantly higher than the model group, MDA content the impact of significantly lower than model group (p<0.05), treatment group 1 in rat liver tissue SOD activity than 2,3-treatment group, MDA content in the film than the treatment group 2,3 (p<0.05); treatment of a group of rat liver tissue SOD activity decreased, MDA content increased with the treatment of 4 groups were not significantly different (P<0.01).Bugan Decoction on rat gastrocnemius muscle SOD activity, MDA content.Treatment group 1 decreased activity of rat gastrocnemius muscle SOD, MDA content increased with no significant difference between the normal group (p<0.01); treatment group 1 on SOD activity of rat gastrocnemius muscle was significantly higher than the model group, MDA content significantly lower than model group (p<0.05), treatment group 1 on SOD activity of rat gastrocnemius higher than treatment 1 group, MDA content higher than 2,3-treatment group (p<0.05); treatment group 1 pairs decreased activity of rat gastrocnemius muscle SOD, MDA content increased with treatment no significant difference between group 4 (P<0.01).ConclusionThe results show that:Bugan soup can of exercise-induced fatigue through improved behavioral performance in rats, inhibition of apoptosis of peripheral blood lymphocytes, serum AST, ALT elevation, exercise-induced fatigue in rats with chronic liver and gastrocnemius morphology Symptoms, liver, gastrocnemius muscle SOD activity, MDA content, Bugan soup rats can effectively alleviate the symptoms of fatigue, chronic fatigue syndrome have a good effect. Chronic Fatigue and clear as the relative specificity of liver stores... |
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