Spinal Cord Injury Patients With Advanced Olfactory Ensheathing Cell Transplantation On Sympathetic Skin Response Clinical Study

Objective:To Observe in patients with advanced spinal cord injury after transplantation of olfactory ensheathing cells of sympathetic skin response latency changes and amplitude changes in ASIA scores, analysis and value of its after effects.Method:①: PARTICIPANTS: 43 patients with advanced spinal cord injury patients, 36 males and 4 females, mean age (30.7±10.3) years, mean disease duration (4.7±3.5) years; complete in 17 cases, 26 incomplete cases, injury segments: cervical 13 cases, 14 cases of thoracic and lumbar 16; cause of injury: car accidents, falls, injured. preoperative can lead to SSR. of both lower extremities to reduce sweating in varying degrees, dark skin color, scaling symptoms.②Cell culture and identification: Take 4 to 6 months embryo induction medium (through the Hospital Ethics Committee approval) of the olfactory bulb, after stripping membrane mechanical manipulation, cultured in the presence of 10% -15% fetal calf serum D/F-12 Training liquid (GIBICO products) in, 3d after the purification of serum-free DMEM culture medium was changed every 3d semi-liquid 1, continue to develop 7-10 d. Digestive cells and made into single cell suspension, the cell concentration of 1×1010L-1 or so. The purified p75 antibodies in cultured cells selected for immunohistochemical staining, positive for olfactory ensheathing cells, the purity of 95% to 98%.③Surgery and cell transplantation: preoperative routine MRI, according to MRI to determine the site of spinal cord lesions. Segmental lesions anesthesia after midline approach to remove the spinous process and lamina of the lesion, dura cut to expose the spinal cord. With the operating microscope in the spinal cord and spinal cord lesions at the junction of the normal part of multi-point injection disposal of approximately 100μL suspension of olfactory ensheathing cells, about 1×106个olfactory ensheathing cells, tightly close the dura mater and suture layers. Given after surgery to stop bleeding, anti-infection treatment④Functional evaluation: the same conditions in the downstream SSR detection, using ASIA motor and sensory table rate before and after surgery were compared before and after the change.Results:All patients after 2 weeks of their lower limb motor, sensory, autonomic function were improved to some extent, improve the performance of autonomic function in varying degrees of skin increased sweating, color improvement, scaling reduced. SSR latency and amplitude measured after its better than before surgery, the incubation period amplitude .The spinal cord motor and sensory function after surgery have significantly improved scores .Conclusion:Olfactory ensheathing cell transplantation for the treatment of spinal cord injury patients, the patients had improved motor and sensory autonomic functions, SSR detection can be more objectively reflect autonomic functional recovery after spinal cord can be used as supplementary ASIA functional assessment of the spinal cord Evaluation of autonomic function. More accurate and comprehensive reflection of cell transplantation for spinal cord nerve function changes.