The Research On The Cochlear Three-dimensional Reconstruction Of Guinea Pig And The Numerical Simulation Of Basilar Membrane

Objective:1.Based on the organization's serial section and combined with finite element software to realize cochlear three-dimensional reconstruction , Making macro anatomical structure display clearly, the structure and function of organism interactive mechanism and morphology, in clarifying anatomy and other areas of research has the vital significance.2.Through three-dimensional reconstruction of the cochlea, not only can accurately display the complex anatomy of the cochlea, and the rotation of the multiple perspectives, obtain length and Angle of area, volume and precise anatomic parameters, For inner ear related diseases pathophysiology, pathogenesis and other basic research, but also for clinical choose the best surgery path and electronic cochlear implant development and provides a quantitative analysis platform.3.Through three-dimensional model of the reconstruction of the cochlea, so that more intuitive to understand and master the cochlea and adjacent structures and even the relative position of the head, but also for the further implementation of other tissues and organs of the three-dimensional reconstruction provides a method reference to the local as well as tissues and organs.4.Based on the cochlear Three-dimensional reconstruction and the finite element specialized software, establish the cochlea basement membrane numerical model. Discusses the structural features of cochlear basilar membrane and modal analysis, numerical simulation for inner ear function provides the research foundation.Materials and Methods: Healthy adult guinea pigs 1, line power otoscopy, auditory brainstem response, otoacoustic emissions and the thin inner ear CT scan, other than ear disease and inner ear malformations, rapid decapitation after anesthesia left out temporal bone, open to listen to bubble, exposing the cochlea and perfusion fixed, the trim, decalcified, dehydrated, transparent, dipping wax, embedding, orientation and other steps underwent serial sectioning and paraffin sort, slice thickness of 0.6×10-5 m, fixed After staining, were mounted. Continuous recording of the slice, a set of inner ear tissue sections obtained image data, the image into Photoshop software, to go to color, gray scale and contrast, and adjust the treatment, the use of software from compiling mimics recognition and demarcation of the border to obtain the boundary points three-dimensional coordinates, the coordinates into Hypermesh software to establish the grid model, using finite element software ANSYS reconstruction, cochlear membrane structure by three-dimensional finite element model, finite element method, solving the simplified model of the basement membrane model.Results:1.Through the organization of consecutive paraffin sections and camera, to obtain a set of two-dimensional serial sections of guinea pig image information.2.Using the paraffin and serial sections of the finite element software, the establishment of a three-dimensional finite element guinea pig model, which can clearly show the characteristics of the spatial structure of the cochlea, with real meaning and value.3.established the three-dimensional reconstruction of the baseilar membrane model, using ANSYS software to solve the baseilart membrane model, obtained the guinea pig baseilar membrane top ten models.Conclusion:1.based on organizational serial section and three-dimensional reconstruction, reflect the detail of the anatomic structure and spatial characteristics, is a way to improve the level of the cochlea and temporal bone morphology of the method.2.Through the organization of Continuous paraffin section through and with ANSYS software, to achieve three-dimensional reconstruction of the cochlea, for the numerical model of structure and function of cochlear relevant interaction mechanism provide the foundation.3.According to the cochlea basilar membrane inherent dynamics properties, combined with organization serial section and ANSYS software, established the cochlea basilar membrane, through solving the modal, deepened to the basilar membrane role in the auditory transduction process understanding of the mechanism of action ,for the future of inner ear disease pathogenesis provides a quantitative analysis platform.