Thymidine Kinase In The Cytoplasm Of The Significance Of Hematological Malignancies

Objective To investigate the changes of serum cytoplasmic thymidinekinase (TK1) levels in patients with hematological malignancies (leukemia, lymphoma, myelodysplastic syndrome and multiple myeloma), and its role on diagnosis and pronosis, especially the relationship among the white blood cell count, hemoglobin level, blood platelet count, serum white protein, serum lactate dehydrogenase, serum C-reactive protein to the TK1 levels detected in the patients.Methods From March 2010 to December 2010, a total of 40 patients with different hematologic diseases was hospitalized to our department. Among those patients, 25 patients were diagnosed to have hematological malignancies, including 9 cases of leukemia, 7 cases of lymphoma, 5 cases of myelodysplastic syndrome and 4 cases of multiple myeloma, the other 15 cases with non-hematological malignancies served as negative controls. In the meantime, patients with other malignancies (34 cases of gastrointestinal cancer, lung cancer in 23 cases, 14 cases of other malignant tumors) were selected as a positive controls. Chemoluminescence dot-blot assay was employed to determine the concentration of TK1 in peripheral blood of those patients. To explore the effects of TK1 levels on diagnosis and prognosis of patients with different cancer, the changes of TK1 in serum was compared with peripheral blood count or other biochemical prolifes..those with the TK1 levels higher than 2.0PM / L was considered as positive cases. The sensitivity and specificity of TK1 in the different diseases were also determined.Results there was no significance of TK1 levels changes among the patients'gender, age, serum albumin, serum lactate dehydrogenase, serum C-reactive protein, white blood cell count and hemoglobin level in group of hematological malignancies, but the high of levels TK1 was documented in those with higher peripheral blood platelet count. PLT≥100*10~9/L,the concentration of TK1 is 1.37±0.89PM/L, while PLT<100*10~9/L,it is 2.37±1.40PM/L. In comparison with gastrointestinal tumors or lung cancers, the changes of TK1 levels in hematological malignancies seemed to be more sensitive and might be used to monitor the changes of the disease during chemotherapy.Conclusion The changes of TK1 levels in hematological malignancies seemed to be more sensitive, and its levels is related to the changes of peripheral blood platelet numbers...