Estrogen Sulfate Transferase (SULT1E1) Expression In Breast Cancer Tissues And Its Clinical Significance

Objective: To investigate estrogen sulfate transferase (SULT1E1) expression in breast cancer, and the relationship between estrogen receptor and its clinicopathological features.Methods:Reverse transcription polymerase chain reaction (RT-PCR) and immunohisto- chemistry were used to detect estrogen sulfate transferase (SULT1E1) mRNA and protein expression, and immunohistochemical method to detect breast cancer in estrogen receptor (ER) protein expression.The expression of SULTlElmRNA in breast cancer tissue, adjacent normal breast tissue and breast fibroadenoma tissues were (0.47±0.30), (0.69±0.30) *, (0.74±0.60)# respectively (* and #, were campared to the breast cancer group respectively, p <0.01). The expression of SULTlElmRNA in the early (Ⅰ+Ⅱ) breast cancer (0.45±0.22) and in advanced (IIIA + IIIB + IIIC) breast cancer (0.52±0.5)(P> 0.05). The expression of SULTlElmRNA in well and poorly differentiated breast cancer in the relative levels were 0.47±0.17; 0.46±0.31,the differences had no stastistical significance (P> 0.05). The expression of SULTlElmRNA in diameter <3cm group of breast cancer (0.49±0.27),in diameter> 3cm group expression (0.43±0.33)(P> 0.05). The expression of SULTlElmRNA in breast cancer With lymph node metastasis (0.48±0.26) and the shift of breast cancer without lymph node (0.45±0.33) compared to no significant difference (P> 0.05). The expression of SULTlElmRNA in premenopausal and postmenopausal breast cancer patients were (0.44±0.27), (0.48±0.32)respectively(P> 0.05).Breast cancer, adjacent normal breast tissue and fibroadenoma tissue SULTlEl protein expression rates were 48.6% (18/37), 81.1% (30/37) * 90% (9 / 10), * respectively. and * was compared to the breast cancer p <0.01,was compared to the adjacent normal breast tissue P> 0.05).SULTlEl protein in the ER receptor-positive breast cancer rate (7 / 21 33.3%), was lower than the ER receptor negative breast cancer (11/16 68.75%), the difference had stastistical significance (P <0.05). SULTlEl protein expression of breast cancer with lymph node metastasis (7 / 18 38.9%) and the shift of breast cancer without lymph node metastasis (11/19 57.9%) compared to no statistically significant difference (p> 0.05).The SULTlEl protein expression in breast cancer tissue with tumor diameter> 3cm (6 / 19 31.6%)was lower than the tumor diameter <3cm (12/18 66.7%), (P <0.05).Conclusion: 1.SULTlEl protein in normal breast tissue, breast fibroadenoma tissue and cancer tissue expressed in the cytoplasm.The expression of SULTlElmRNA and protein in breast cancer are lower than normal breast tissue, the differences had stastistical significance (P <0.05). The SULTlElmRNA and protein levels of ER-positive breast cancer are relatively lower than the ER-negative breast cancer, the expression of SULTlElmRNA and protein in ER-negative breast cancer tissue and normal breast tissue are similar. Tip SULTlElmRNA and decreased protein expression may be related to the occurrence of the hormone-sensitive breast cancer .