Magnetic Activated Cell Sorting In Vitro Separation Method Of Rhesus Monkeys Bone Marrow CD34+Cells And Induction Method Of DC And Identification

[Object]By magnetic activated cell sorting in vitro separation method of rhesus monkeys bone marrow CD34+cells, reuse cell factors to induce DC directional differentiation and identify it of the surface marks and morphology.[Methods](1)Acquisition bone marrow samples:take rhesus monkeys on the anterior superior iliac spine or under the after iliac2-3cm bone marrow puncture for bone marrow.(2)Bone marrow separation of mononuclear cell:fresh bone marrow specimens, diluted volume, slowly add monkey lymphocyte separation liquid surface, after liquid horizontal centrifugal visible from up to down points four layers, will be separated the layer of mononuclear cell with ivory(3)The magnetic activated cell sorting isolate the method of bone marrow CD34+cells:using immune magnetic beads kit and screening positive CD34+cells.(4)Induced CD34+cells to DC differentiation.(5)Use flow cytometric, electron microscope to respectively identify DC of the surface marks, internal structure and the external form.[Result]Successful induction of rhesus monkeys bone marrow imDC, mDC and identification.[Conclution]We get the rhesus monkeys imDC of bone marrow, for a stage of building before PD-LlIg and CD40L exocellular area double gene adenovirus particles modify and infection of imDC have laid a foundation and push forward one step furtheror for the final study rhesus monkeys liver transplantation immune tolerance;...