Preliminary Study On Effect Of11Natural Diterpenoids To Inhibite Bladder Cancer In Vitro

bjective:Bladder cancer is one of the most common malignancies. tumor recurrence is a major clinical concern. Unfortunately, current chemotherapies are insufficient in bladder reperfusion.Therefore, there is an obvious urgent need for novel, effective and safe therapies to prevent both recurrence and progression. According to study on effect of11natural diterpenoids to inhibite bladder cancer in vitro, we can screen a new chemotherapy of bladder cancer.Method:To obtain the best consistency of bladder cancer T24cells in vitro, Using MTT colorimetric method and taking MMC as the positive control to research the inhibitory effects of11antitumor compounds to the bladder cancer in vitro:SA-1, S-3,CM-6,S-11,S-4,S-1,CM-4,S-12,CM-3,S-5and SR-37on the growth of T24cells and its value of IC50with40,8,1.6,0.32,0.064μmol/L five different concentration in different hour24h and48h. Firstly, taking20μmol/L concentration of each sample as preliminary screening. Based on the result of the preliminary screening, we set40,8,1.6,0.32,0.064μmol/L screen again to detect the OD of11diterpenoids to inhibite the bladder cancer in vitro in24h and48h, then figure up each IC50with relevance formula.Result:The IC50of MMC. SA-1,S-3,CM-6,S-11,S-4,S-1,CM-4,S-12, CM-3,S-5,SR-37to the T24bladder cancer cell in48h as follows:2.55,6.16,6.64,8.87,6.06,3.79,8.25,6.69,7.94,8.33,10.46μmol/L. There are10kinds of compounds which values of IC50are less than10ug/mL. S-4has the lowest value of IC50to the T24bladder cancer cells.Conclusion:Among11compounds. S-4has the highest value of IC50to the T24bladder cancer cells. The inhibition rate of the11compounds to the T24bladder cancer cells assume time dependence and concentration dependence. By preliminary study on inhibitory effect of11natural diterpenoids to bladder cancer in vitro, S-4had layed foundations in treatment of bladder cancer patients.