Experimental Study Of Neovascularization Of Transplanted APD Area Promoting By Erythropoietin Mobilizated Endothelial Progenitor Cells

Objective:To observe erythropoietin's effect on the mobilization of EPCs (endothelial progenitor cells) in the bone marrow and transplanted APD area neovasculorization. To select optimizing mobilization methods and occasions and p rovide some new ideas and simple method for vascularizing transplanted tissue engineering skin(TES) and other artificial stent rapidly.Methods:Phase I:According to the literature described dose (EPO dose of1000IU/Kg*d-1and3000IU/Kg*d-1) and methods(continuous intraperitoneal injection of7days), EPCs was mobilized by Recombinant Human Erythropoieti-n injection (ShanXi Weiqidaguang PharmaceuticalCo., Ltd) in mice.108healthy male Kunming mice(avoiding the interference of estrogen) were randomly divided into group A (low-dose EPO mobilization group), group B(high-dose EPO mobiliza-tion group), groupC (NS control group), each group was36mice.6mice was randomly selected in each group after mobilization Id,3d,5d,7d10d,14d, Flk-1and CD133positive cell rate of selected mice heart blood was detected by FCM(flow cytometry), the results, said as Rank mean, are statistics compared and analyzed. Phase II:Continuous intraperitoneal1000IU/Kg*d-1EPO injection of7days, EPCs was mobilized by Recombinant Human Erythropoietin injection (ShanXi Weiqidaguang PharmaceuticalCo.Ltd) in mice. On the7d mobilization, acellular pig skin was transplanted subcutaneously in mice's back. 48Healthy male Kunming mice(avoiding the interference of estrogen)were randomly divided into group A (EPO mobilization group), group B (NS control group), each group was24mice.6mice were randomly selected in each group after mobilization3d,7d,14d. Flk-1and CD133positive cell rate of selected mice heart blood was detected by FCM(flow cytometry), the results said as Rankmean are Statistics compared andanalyzed. The pathological morphology and angiogenesis (marked by CD34) of APD transplanted area was observed at transplanted3d,7d,14d,21d. The results, said as χ±s, are Statistics compared and analyzed.Results:Phase I:Group C (control group) double fluorescent positive cells were not significantly different in6time point (P>0.05).The double fluores-cent-positive cells of GroupA (low-dose EPO mobilization group) and group B (high-dose EPO mobilization group) gradually increased and peaked at7d. From3d, in each time point the proportion of double fluorescence positive cells of three groups have significant differences (P<0.05); double fluorescent positive cells of Group B, compared with group A and group C, increased significantly (P<0.05); double fluorescent positive cells of group A, compared with group C, increased signifycantly(P<0.05). Mobilizated EPCs in the peripheral blood results in a dose depend-entmanner by EPO. Phase II:In the three time point double fluorescent positive cells of group B (control group) were not significantly different (P>0.05). Double fluorescent positive cells of Group A (EPO mobilization gro-up) gradually increased and peaked on7d. In each time point double fluorescent positive cells of group A, compared with group B, increaseed (P<0.05). EPCs promote angiogenesis of acellular porcine dermis transplanted area is significantly stronger than control group by EPO.Conclusion:The number of peripheral blood EPCs increase and reached a peak on the7d by intraperitoneal7d EPO injection, The EPO mobilization effect is effective in a dose dependent. Intraperitoneal EPO injection promote neovasculorization of acellular porcine dermis transplanted area.