The Research On The Protective Effects Of Resveratrol Against Homocysteine-induced Oxidative Injury In PC12Cells

Objective In the present study, we aim to investigate the protective effects of resveratrol (Res) on homocysteine (Hcy) induced oxidative injury model of PC12cells (the rat adrenal gland pheochromocytoma cell line).Methods PC12cells were cultured and treated in vitro. During the experiments, PC12cells were randomly divided into three groups, including the blank control group, the Hcy treatment group and the Res treatment group. Intracelluar ROS was measured by spectrofluoro-photometer using H2DCFDA. Quantification of cell death was observed by Hoechst33342/PI nuclear staining and fluorescence microscopy.Results3～8mM Hcy could induce significant increase of PC12cells' intracelluar ROS as well as apoptosis and necrosis in a concentration-dependent manner.5mM Hcy could induce significant increase of PC12cells'intracelluar ROS as well as apoptosis and necrosis in a time-dependent manner. The production of intracellular ROS induced by5mM Hcy of PC12cells was inhibited by5μM～25μM Res in a concentration-dependent manner.5～25μM Res could inhibit apoptosis and necrosis of PC12cells induced by5mM Hcy in a concentration-dependent manner.Conclusions Hcy could induce apoptosis and necrosis of PC12cells by increasing intracellular ROS in time-dependent and concentration-dependent manner. Resveratrol was found to be protective against oxidative stress as well as apoptosis and necrosis induced by Hcy in PC12cells.