Extraction, Purification And Pharmacological Research Of Lotus Leaves Alkaloid Salt

Currently, health issue has become one of the biggest concerns of people around the world. Green food made by natural product has come to the eyes of the public. Lotus leaves, abundant in our country, are considered natural nourishment which can be both served as food and medicine. Fully exploring the values in medicine and food is very important.This paper was carried out using lotus leaves as materials. The optimal extraction and purification of alkaloid salts were obtained through acid water macroporous adsorption resin. The antioxidant ability of product and Hypolipidemic effects were studied with the results as follows:The hot refluxing acid water extraction of alkaloid salt from lotus leaves were compared. The optimal extraction was established by UV-Vis spectrophotometer and High Performance Liquid Chromatography. The conditions for hot refluxing extraction was optimized by single factor and response surface method:extraction temperature92.7℃, extraction time2.28h, the solid-liquid ratio1:24.4(g/mL), the concentration of phosphoric acid0.27%。the total alkaloid salt extracted in this conditionwas5.313mg/g.Alkaloid salts from lotus leaves were purified by macroporous absorption resin. The best resin was D101screened by static method. The purification was optimized by dynamic method:concentration of alkaloid salts was2.352mg/mL with lmol/L NaCl, flow rate2.5BV/h, concentration of washing solution70%, elution rate2.0BV/h and the purity of nuciferine salts was3.5%.Three assays were used to evaluate the antioxidant activity of alkaloid salts from lotus leaves. The results indicated:the total antioxidant activity of1g acid extracts of lotus leaves was equivalent to0.0429gVit C while1g purified sample was equivalent to0.1488gVit C; The reducing power of1g acid extracts of lotus leaves was equivalent to1g acid extracts of lotus leaves was equivalent to 0.0403gVit C while1g purified sample was equivalent to0.1539gVit C; While1g purified sample was equivalent to0.1488gVit C; The scavenging rate of DPPH increased with the increasing of crude extracts and purified sample when they reach2mg/mL and0.434mg/mL, with the IC50value of0.225mg/mL and0.063mg/mL, respectively.The fat lowering of alkaloid salts from lotus leaves was evaluated using SD rats as experimental subject. The result indicated:when the rats were fed with high fat emulsion, the TG,TC and AI were significant higher that control group. Alkaloid salts from lotus leaves could significantly decreased TC,TG and non-HDL-C of the high fat emulsion group and meanwhile, increased HDL-C. Alkaloid salts from lotus leaves can significantly decreased the risk of atherosclerosis of SD rats in model groups. The high alkaloid salts group can effectively decrease MDA in hepatic tissue and TG,TC index.