Preliminary Study In Vitro And In Vivo On Allograft Rabbit Adipose-Derived Stem Cells Combined With PGA/PLA To Build The Tissue Engineeirng Of Fat

Objective:To explore the possibility of building tissue engineering of fat with allogeneicrabbit adipose stem cells(rADSCs) combined with PGA/PLA.Methods:1. Using trpe Ⅰcollagenase digestion, ADSCs were isolated frome the fat ofrabbit and then primary culture and to observe the growth of rADSCs;2. With0.25%trypsin-EDTA digestion and subcultured and observed;3. The passage3rADSCs underwent adipogenic,osteogenic and chondrogenicinduction and identification;4. Preparation of PGA/PLA;5. The third generation of rADSCs combined with the preparation of PGA/PLAmaterial, after2weeks of adipogenic induction,transplanted to the left side of therabbit back(experimental group);the simple materials group of the same conditionsafter inducted transplanted to the right side of the rabbit bact(control group),the totalof16groups;8weeks later implants were taken out and general observation and HEstaining were used for morphological observation.Results:1. The sources of rADSCs are widely, culture technology is mature rADSCscould proliferate rapidly in vitro;2. Passaged mature technology and operation simply and then rADSCs arepurified;3. ADSCs of rabbit have adipogenic,osteogenic and chondrogenic potential invitro; 4. Preparation of PGA/PLA is simple from using PGA,PLA by a certainpercentage, easy to prepare;5. Adipose tissue-like new-born tissues were found in the expperimental group,the average wet-weight of them was (14.08±0.90)mg, residual material were foundin the control group, the average wet-weight of them was (8.91±0.40)mg,thedifference between the two groups there are statistically significant,P＜0.05; HEstaining confirmed that most of the new-born tissue of the experimental group wasadipose tissue,while most of the new-born tissue of the control group was fibroustissue.Conclusion:Allogeneic rADSCs combined with PGA/PLA can build fat tissue engineering.