RNA Interference On PANC-1 Pancreatic Cancer Cells HTERT Gene Expression And Cell Apoptosis In Value-added

Objective:PANC-1pancreatic cancer cells transfected by recombinant plasmid pcDNA6.2-GW/EmGFP-miR-hTERT-siRNA, preliminary observation on cells in vitro anti-cancer of the pancreas.Method:PANC-1cells transfected by Lipofectamine2000recombinant plasmid pcDAN6.2-GW/EmGFP-miR-hTERT-siRNA Positive cells by Blasticidin S HCl,scre-ening two weeks, extraction of RNA by RT-PCR.Results:RT-PCR results show that:Transfectedl31,141pieces of interference PANC-1cell hTERT-mRNA expression of the NC is negative control and not turned the plasmiid PANC-1cells of the hTERT-mRNA control expression level down significantly.At the same time NaCan control GAPDH,no obvious difference between the cells.Conclusion:restructuring plasmid pcDNA6.2-GW/EmGFP-miR-hTERT-siRNA can specificity induction homologous complementary mRNA degradation,cut hTERT mRNA express, have good RNAi silence effect. Objective: PANC-1pancreatic cancer cells transfected by recombinant plasmid pcDNA6.2-GW/EmGFP-miR-hTERT-siRNA, Observe the PANC-1for pancreatic cancer cell apoptosis and value-added role.Methods:Through the has successfully transfected recombinant plasmid pcDNA6.2-G W/EmGFP-miR-hTERT-siRNA pancreatic cancer cells PANC-1, after48hours to collect cells, the Annexin V, PI and Brdu dyeing, application flow cytometric observation cell apoptosis and appreciation.Results:streaming results show that:ZhuanRan112,121,131,141pieces of interfer-ence PANC-1cell apoptosis is NC late negative control and not turned the plasmid PANC-1cells are significantly increased, but no significant effect of early apoptosis, to transfecte112.121.131,141pieces of interference PANC-1of the value of the cells are the influence of different level, including131,141more obvious influence of appreciation.Conclusion:hTERT siRNA interference can through the specific inhibition to suppr-ess tumor cells hTERTmRNA express telomerase expression, and then reach the in vitro inhibit pancreatic cancer cell growth and proliferation, and promote the role of pancreatic cancer cell apoptosis, tip hTERT mRNA telomerase activity is the important factors, and lowers the telomerase activity to treat pancreatic cancer reliable site.