Effects Of Dragon Blood And Loureirin B Monomer On Hepatic Stellate Cells Promoting Angiogenesis And The Influence Of Liver Fibrosis

Part I:Loureirin B on experimental hepatic fibrosis and its possible mechanismObjective:To investigate Loureirin B on experimental hepatic fibrosis, and present possible mechanisms。Method:36clean male SD (Sprague-Dawley) rats were randomly divided into4groups:Control group, model group, drug treatment group; With thioacetamide (TAA) inducing liver fibrosis model, to bserve serum transaminase (ALT, AST), fibrosis markers (HA, LN, IV-C), liver histopathology changes.Results:To compare with model group,(1)liver pathology grade, Control group were more serious than the model group; drug treatment group were better than the model group;(2) The level of drug treatment group ALT, AST decreases significantly (P<0.01),(3) The level of drug treatment group HA, LN, IV-C is significantly decreased (P<0.01)。Conclusion:Rat liver fibrosis model induced by TAA is established Successfully; dragon's blood can protect TAA-induced liver fibrosis in liver function, and improve the quality of life in rats with liver fibrosis; dragon's blood treatment on liver fibrosis may be positively correlated with the dosage. Part Ⅱ:Effect of Loureirin B on Proliferation and Extracellular Matrix Secretion of Rat Hepatic Stellate Cells in Vitro Objective:To investigate the effects of loureirin B on proliferation and extracellular matrix secretion of rat hepatic stellate cells in vitro.Methods:HSC-T6was cultured in96-well plates for24hours.Then they were incubated with different concentration of loureirin B for48hours.MTT colorimetry was used for assaying proliferation of hepatic stellate cells.lnhibition rate was calculated. Hyaluronic acid Laminin and Collagen type IV were measured by radioimmunoassay.The expression of vascular endothelial growth factor hypoxia-inducible factor and neuropilin-1mRNA.Results:Addition of loureirin B into culture medium could significantly inhibit HSC proliferation,and the higher concentration of the loureirin B the stronger inhibition rate of the hepatic stellate cells. It also inhibit Hyaluronic acid,Laminin and Collagen type IV secretion in different degree. The expression of vascular endothelial growth factor hypoxia-inducible factor and neuropilin-1mRNA.Conclusion:Loureirin B can significantly inhibite hepatic stellate cells proliferation and extracellular matrix secretion. And inhibite the expression of vascular endothelial growth factor hypoxia-inducible factor and neuropilin-1.