Influences On The Essential Oil's Chemical Substances Of Aquilaria Sinensis (Lour.) Sprengel Disposed By Different Methods Of Artificial Fungus Inoculation

The author had studied the the formation of agilawood in the Aquilaria sinensis (Lour.) Sprengel disposed by different methods of artificial fungus inoculation. The health plants of Aquilaria sinensis were disposed by two methods, the fungus in solid status and in liquid status, and there were sealed and unsealed holes in each method. Then the writer extracted the essential oil from the xylem of A. sinensis every month in a half of year and untreated plants with absolute aether. The chemical constituents of samples were analyzed by GC-MS.The results are as follows:1. The essential oil of untreated plants was analyzed by GC-MS and its chemical constituents were heteroaromatic compounds, steroid compounds, aliphatic acid compounds and terpenes. The main chemical compounds of essential oil in this experiment were clionasterol(23.487%) and Coniferol(10.228%)2. The writer extracted the essential oil from the samples which were disposed by solid status and sealed. The extraction ratio was1.474%, which were higher than the extraction ratio using the same method of artificial fungus inoculation in air. The writer thought the way in this study could improve the extraction ratio.3. The extraction ratios of disposed samples were higher than the one of undisposed samples and the extraction ratios of disposed samples rose gradually. At this process, the author could detect the agilawood's characteristic chemical compounds. The result showed that the plants of Aquilaria sinensis would form something liposoluble and defensive agilawood when they encountered mechanical damages and fungal infections.4. The writer detected some compounds which couldn't be found in the agilawood saled in the market, such as the alkene compounds and steroid compounds. Parts of the aromatic and sesquterpenoid compounds which could be detected in the samples had fluctuated changes in the absolute contents. The writer conjectured that all these compounds above were the intermediate products in the process of agilawood in the plants of Aquilaria sinensis.5. Comparing the essential oil's extraction ratios and total absolute contents of agilawood's characteristic compounds in the sixth samples from the samples, the author found the absolute content from the samples disposed with the fungus in solid status and the holes unsealed, was the highest. So the writer conjectured this method was the best one.