Study Of The NF-κB Inhibitor Pyrrolidine Dithiocarbamate (PDTC) Inhibits Aseptic Loosening Of Prostheses Induced By Wear Debris

Objectives：To study the effect of NF-KappaB inhibitor pyrrolidine dithiocarbamate(PDTC)on aseptic loosening induced by wear debris, to provide a theoretical basisfor the prevention and treatment of aseptic loosening around the prosthesis.Method：Prepare5mg/ml metal wear debris of artificial joint solution suspended innormal saline and air pouch mouse model. Inject the suspension into the pouch.30Kunming mice (mouse bone implant air pouch model) are randomly put into3groups:positive control group is injected with wear particle suspension in the pouch andnormal saline in the abdominal cavity; test group is injected with wear particlesuspension and PDTC (abdominal cavity) respectively; negative control group isinjected with normal saline in both. Execute all test subjects and acquire the bonefragments in the air pouch. Each sample divided evenly into two parts: one is sent forRT-qPCR to examine the expression of inflammatory factors, while the other is fixedby formaldehyde and prepared as paraffin sample for HE staining and TRAP stainingto specifically detect the osteoclasts.Results：Inflammatory cell count in positive control group, test group and negativecontrol group are statistically different,(7305±797/mm2,5651±694/mm2and3459±424/mm2respectively, P<0.05); Statistically difference in TRAP stainingpositive cells in microscopic area occupied in the3groups has also beenobserved(4.220%±1.440%,1.192%±0.124%,0.760%±0.142%,respectively, P<0.05);IL-1β and MMP-9expression in the mice in each group show a decreasing differencebetween the two groups were statistically significant (P<0.05).Conclusion： According to the results of this study, we believe that NF-KappaBinhibitor PDTC can inhibit wear particle induced expression of inflammatory factorexpression and the proliferation and differentiation of osteoclast, thereby suppressingosteolysis process.It plays a role in prevention and treatment of aseptic loosening ofthe prosthesis. This study provide experimental evidence for prevention of artificialjoint aseptic loosening.