Studies On Ganfukang Capsule And Its Effects On Human Hepatocellular Carcinoma Cell Line HepG2

Ganfukang Capsule is a kind of Chinese medicine capsules made Ganfukangpill according to it formulations reform (change the extraction process and dosageforms). Ganfukang pills is the ministerial standards of Chinese medicine prescriptionpreparation7reproduces traditional Chinese medicine prescription, preparations bySchisandra, Taizishen, Hedyotis diffusa shamisen, traditional Chinese medicine. It aneffective Chinese patent medicines for the treatment of hepatitis, the favorableresponse, for acute and chronic hepatitis, early cirrhosis and liver dysfunction,because has the convergence of supplementing Qi, detoxification, lowering alanineaminotransferase. But the original formulation has inadequacies as following：1.thepreparation process is backward: Since the original formulation of pills, herbs crushmade pills, the preparation process is very backward;2.the quality control standards isbackward: quality standards of the original pill only has two test-tube reactions;3.taking in large dose: Since the original pills crush the pill, the medicinal productcontains a large number of non-medicinal ingredients, thus the taking dose in large,6~9g per time, the patient is more difficult to accept. Intends to prescription ofChinese herbal medicines made into capsules were extracted, can significantly reducethe intake of non-medicinal ingredients, patients taking convenience. In addition, byMTT assay, immune cells, chemical and flow cytometry techniques from molecularcell biology, immunocytochemistry to investigate the Ganfukang capsule cancer orpossible ways of blocking malignant transformation. provide a treatment for clinicalhepatitis, liver cancer, and easy to take Traditional Chinese Drug Research. The maincontents and results of the study are as follows:1. The extraction process: use the orthogonal test, the transfer rate to the totallignan examine alcohol extraction process, butanol extract and total saponinsinvestigated as an indicator of boiling process, the experimental results indicate thatSchisandra5times the amount of percentage70by ethanol extraction, every twohours as excellent, Taizishen, and Hedyotis diffusa boiling for3times uesing8timesthe amount of water, two hours every time for the gifted.2. The molding process: the particle mobility, moisture absorption, pile density, and disintegration time as an indicator, study the preparation of the prescription thepros and cons. Drawn Ganfukang capsule molding process to take dry extract, adding(in this case refers to the quality of20percent or volume or other)20%starch,15%silica powder and5%carboxymethyl starch sodium, mix to80%ethanol, granulation,drying, whole, to fill capsules.3. Ganfukang capsule quality standards: the right the shamisen medicineprescriptions in the new formulation quality standards have established a thin layer ofthe qualitative identification by HPLC method for the determination of thepreparation Schisandrin; the quality standards moreeffective control of productquality, improve the controllability of the quality of the product.4. Ganfukang capsule stability studies: through to observe the law at roomtemperature and to stay at room temperature on retained samples observation methodinvestigated: its appearance, identification, capsule weight variation, disintegrationtime and content of the results are in line with the quality standards of theproductshealth examination for compliance Description Ganfukang capsule isbasically stable during the stability test tentatively scheduled to be valid for twoyears.5. Chinese medicine Ganfukang capsule of the human hepatoma cell line HepG2:MTT assay was used for the determination of of Ganfukang capsule on theproliferation of human hepatoma cell line HepG. Flow cytometry was used to analyzethe cell cycle, apoptosis, DNA relative content of the observed liver cancer cells,analysis of cell cycle changes in each period. Induced the expression of apoptoticprotein Omi/HtrA2and Casepase-3in the detection of drug action usingimmunocytochemical methods. The results showed that the liver Rehabilitationcapsule has good inhibition in HepG2cell hyperplasia in rats, showing the time-adose-dependent; applications liver Rehabilitation capsule increased proportion ofHepG2cells in G2phase, G1and S phase cells decreased, and the apoptosis rateincreased.