T-mTOR And P-mTOR Expressions In Rat Myocardium At Different Exercise Training Time Point

Objective: to explore the role of exercise in my ocardial function in the process, as well as the impace of the exercise,we observe the expression of the member of t- mTOR p-mTOR.Methods: Model of acute training:we selected 50 male clean SD rats for actute exercise and they were randomly divided into a control group and acute exercise (instantly after exercise, control ,3 hour, 24 hours after exercise), the rats control group just lived a normal life in cafe in cage and don't exercise, after 3 days adaption,the actute exercise training group awere trained on the treadmill which refered to the Bedford increasing load model.Model of a month exercise group: were randomly divided into a control group and Group of a month exerciseing (instantly after exercise, control,3 hour, 24 hours after exercise),in 50 SD male rats. the rats control group just lived a normal life in cafe in cage and don't exercise, after 3 days adaption,the exercise training groups were trained on the treadmill which refered to the Bedford and wangyunhong increasing load model.Model of 3 month exercise group: were randomly divided into a control group and Group of a month exerciseing (instantly after exercise, control,3 hour, 24 hours after exercise),in 50 SD male rats. the rats control group just lived a normal life in cafe in cage and don't exercise, after 3 days adaption,the exercise training groups were trained on the treadmill which refered to the Bedford and wangyunhong increasing load model. Big mouse each week trains six days resting at Sunday, training 10 weeks. the concrete training plan is: the rats control group just lived a normal life in cafe in cage and don't exercise, trains group exerciseing on monday to Saturday, resting on Sunday.we record the body weight on Every Monday.The rats were killed after the last trainging according to the schedual time.following we took out Apex cordis of Left ventricle in the heart. Then the collection protein sample (attrition, raises protein, measures protein, adjusts protein, boils protein, subpackage), the electrophoresis, transfers the membrane, the seal, a anti-foster, two anti-fosters, protein examination.The obtained data counts the software analysis with SPSS13.0.Result: in acute training the expression of t-mTOR and p-mTOR does not have the change on myocardial.A month training group the expression of t-mTOR and p-mTOR on myocardial :the express increased in 3 hours,24 hours after exercise compared with control group. The difference is not remarkable.3 months training group the expression of t- mTOR and p-mTOR on myocardial :the express increased in 3 hours, 24 hours after exercise compared with control group. The difference is remarkable.Conclusion:1 in acute training the expression of t-mTOR and p-mTOR does not have the regular change on myocardial. it indicated mTOR signal passage is not remarkable on myocardial in acute training.2 A month training group the express increased in 3 hours, 24 hours after exercise compared with control group.the express increased in 3 hours, 24 hours after exercise compared with control group. The difference is not remarkable. A month training that did not cause the change of t-mTOR and p-mTOR protein.3 3 months training group the expression of t-mTOR and p-mTOR on myocardial :the express increased in 3 hours, 24 hours after exercise compared with control group. The difference is remarkable.mTOR signaling pathways closely related with myocardial hypertrophy.mTOR signaling pathways may be involved in the regulation and morphological change of myocardium function.