Cell Biology Research On Carcinogenesis Of Mobile Phone Radiation

With the fast development of science and technology, the demand for wirelesscommunication rapidly increase, mobile phones have been widespread used andgreatly improve the quality of life. At the meantime, the mobile phone radiation hasbecome a new environment pollution source which maybe endanger human health. Toprevent from its negative effects on human health, scientists thoroughly study thebioeffect mechanism of mobile phone radiation by epidemiological survey, in vivoand in vitro experiment. However, the results to date give no consistent or convincingevidence of a causal relation between radiofrequency field exposure and cancer risk.This studies explore the carcinogenesis of mobile phone radiation from the cellbiological perspective.The inductive effect of mobile phone radiation on Epstein-Barr virus (EBV)early antigen in Raji cells was primarily studied. Raji Cell line carries the latent EBVgenome and expresses the Epstein-Barr virus nuclear antigen (EBNA). Variety ofantigens as the Epstein-Barr virus early antigen (EBV-EA) can be expressed whenRaji cell is stimulated by chemical or physical carcinogens. Raji cells were dividedinto four groups,3flasks per group. Group A and group B were not exposed to anyradiation. Group C and group D were exposed for4hours to microwaves emittedfrom a GSM900MHz mobile phone in the incubator every day. In the experimentalprocess, the geometric center of the cell culture flask bottom was put over the mobilephone antenna and1mm from the surface of the mobile phone. Theoreticallyspeaking, averaged Specific Absorption Rate (SAR) values in1g were0.020mW/g,and they were performed with the SPEAG DASY5Systems. The radiationexperiments were carried out for4weeks. Group B and group D were cultured in themedium with1ng/mL12-O-tetradecanoylphorbol-13-acetate (TPA) used as tumorpromoter for48hours before each test. Cells were sampled to be tested byimmunocytochemistry with EBV-EA IgG antibodies every week. The experimentresults indicate that, mobile phone radiation will induce the expression of EBV-EAand the induction is even more evident cooperating with tumor promoter such as TPA.The transformation of NIH/3T3cells induced by mobile phone radiation was further carried out. The malignant transformation system of NIH/3T3cells cultured invitro is sensitive to the carcinogen or cancer promoter in environment and is adoptedas a classic method to clarify carcinogen or cancer promoter. The experiments in thisstudy includ continuous and intermittent radiation two ways. During the40-daycontinuous radiation experiments (12hour-a-day), NIH/3T3cells show no changes inmorphology and proliferation. But the study found that the radiation can increase theadhesion strength between NIH/3T3cells and DB Matrigel. During the16-dayintermittent radiation experiments (50mins on/10mins off,10times a day),single-celled gel electrophoresis assay fail to detect DNA damage of induced directlyby mobile phone radiation. Compare with TPA group, the DNA damage induced byTPA cooperating with mobile phone radiation is markedly higher.The experiment show that mobile phone radiation can induce expression ofEBV-EA in Raji cells. While in another experiment, NIH/3T3cells show no changesin morphology and proliferation, however, compare with TPA group, the DNAdamage induced by TPA cooperating with mobile phone radiation is markedly higher.Study reveals the possible existence of a relationship between mobile phone radiationand cancer genesis.