Phenotype Analysis Of Arabidopsis Temperature Sensitive Mutant Tes1

Plants produce different responses with the changes of external environment, and show a series of significant reaction when the ambient temperature occurring small changes, such as germination, flowering, growth rate, and so on. However, the response components of the early temperature signaling pathways is not yet reported. Arabidopsis thaliana as research material, established the plant temperature sensitive mutants screening system, and mutant screening according to the significant differences in plant growth and development under different temperature. A gain-function T-DNA insert muant tesl which phenotype is temperature sensitive isolated.Through the phenotype analysised, as the ambient temperature (17℃,22℃,27℃) increased, tesl shows the hypocotyl length become longer and the root left deflection angle become larger. After analysis the clockwise and counterclockwise gravitropism, the time of tesl root respond to gravity is not changed, but the root left deflection angle of tesl become larger of the clockwise gravitropism seedlings and smaller of the counterclockwise seedlings. With exogenous of auxin, the root left deflection angle of tesl is not changed. Through the starch staining of WT and tesl, there was no difference. After hybridity the tesl and DR5::GFP or Tublin::GFP marker, there was no changes in the root tip fluorescence of the F2generation of tes1♂×Tublin::GFP♀, and become asymmetry fluorescence distribution in the root tip of the F2generation of tes1♂×DR5::GFP♀. In short, the tesl may be a mutant related to the temperature signal and the tropism signal. The research of tesl will help us to understand the changes of the temperature signal how direct action on the plant tropism, and revealing the diversity impact of temperature on plants, and tesl may be a great significance to research how plant feel the temperature signal.