Isolation And Polymorphic Analysis Of Microsatellite Loci For The Omei Treefrog(Rhacophorus Omeimontis)

The choice of female and male to mating partners is common in many animals, which is affected by a lot of factors, such as the size of the body, color, smell, decoration and sperm quality etc internal factors, and field, resources, grade status, temperature and altitude etc external factors. In recent decades, the mate choice has been studied by many domestic and foreign researchers using the methodes of ecology and molecular biology. The species of research included mainly fishes, birds, reptiles and mammals, very few studies focused on mate choice of amphibians. The difference of the number of mating partners led to different mating system that included mainly monogamy and polygamy. Polygamy can be divided into polygyny, polyandry and promiscuity. Mating system can produce important ecological and evolution effect. For example, mating system can affect distribution of genetic variation of within-population and inter-population, and maintain variation in populations, and affect the evolution territoriality and the intensity of sexual selection. The mate choice plays an important role for research in mating system and sexual selection. However, many important questions about the evolution mechanisms of mate choice remain to be answered.In the field study, we find often that the Omei treefrog (Rhacophorus omeimontis) parade a polyandry mating system in a single reproductive period, which one female mate with multiple males. This reproductive pattern indicates that the Omei treefrog is an excellent model for studying the evolution mechanisms of mate choice. Thus, this study describes the development of11novel microsatellite markers for mate choice studies in the Omei treefrog. The number of alleles per locus in24individuals ranged from4to15, the average observed and expected heterozygosity per locus ranged from0.250to0.839and from0.562to0.914, respectively. Two loci showed significant deviations from Hardy-Weinberg equilibrium. Two locus pairs exposed significant linkage disequilibrium. All loci displayed low frequency of null alleles, and no evidence for scoring error or large allele dropout. The reason of null alleles at two loci is because of general excess of homozygotes for most allele size classes. In addition, among11microsatellite loci screened, the PIC of eight sites is greater than0.5, accounting for72.7%of the total locus, which suggests that eight sites are highly polymorphism, they can provide abundant information of sites. These polymorphic loci will be useful markers in studying mate choice of the Omei treefrog.