Molecular Phylogeny Of Chinese Grimmiaceae

Grimmiaceae is a common drought-enduring moss, always grows in the sandy soil androck. According to the report, Grimmiaceae contains12genera in the world, and there are6genera in China. Major of them are distributed in the cold temperature region and thesub-tropical high-altitude areas. Grimmiaceae is the object of study and using morphologyand molecular data of rps4and trnL-F gene of the chloroplast genome as analysisindex.Through the direct DNA sequencing obtained3genera and39taxa rps4and trnL-Fsequence of Grimmiaceae plants. And some sequence of related species were downloadedfrom Genebank for this analysis.The sequences were comparied and analyzed by Clustalx andMEGA5.0bioinformatics software. Phylogenetic trees obtained from the maximumlikelihood（ML）and neighbor joining（NJ）methods. Preliminary analysis results are asfollows：1. In cladistics analysis, although the schistidium is neither recognized as a monophyleticgroup nor closely related to Grimmia.Therefore,we don,t support the point that schistidium isthe subgenus of Grimmia.2. In tree diagram of cladistics, Indusiella, Jaffueliobryum and Ptychomitrium areclustered Grimmia together. Their morphological characteristic is similar as Grimmia, andhave the closer phylogeny relationship.3. In the analysis of phylogeny, as the branch of Grimmiaceae,Racomitrium isrecognized as a monophyletic group, and constitute a sister groups with Grimmia、schistidiumand Coscinodon.4. Grimmia cribrosa and Grimmia alpestri are clustering into Grimmia. In the classicalclassification, their morphology structures are closely resemblant. According to the analysisthat they have highly homologous and closer relationship.5. Based on the molecular datas and morphological analysis, it indicate thatPtychomitrium are closed to Jaffueliobryum and Indusiella.They should belong to thecategory of Grimmiaceae.