Proteome Of Human Peripheral Blood Monocyte

The mission of the international Human Proteome Project (HPP)is to help elucidate biological and molecular function and advance diagnosis and treatment of diseases by systematically cataloguing all the proteins manufactured in the human body-what they are, where they are, and in what abundance. Human peripheral blood monocyte (PBM) is an important cell type, which is involved in various human complex diseases, such as autoimmune deficiency, HIV, cancers, osteoporosis, etc. So far, human PBM proteome has not been tackled by the Human Proteome Organization (HUPO) Initiatives. Thus, a catalogue of human PBM proteins has yet to be established.In this study, through integrating76independent PBM proteome datasets acquired for76unrelated Caucasians, we established the first PBM proteome knowledge base for humans. This knowledge base conveys information of identity of PBM-expressed proteins, protein abundance, protein biochemical features (molecular weight and isoelectric point), and protein functions (including subcellular localization, molecular function, and biological process). Specifically, a total of2,413PBM proteins were cataloged. Functions of these PBM-expressed genes were annotated and classified via Gene Ontology (GO) analysis. The annotation revealed a number of well-recognized-genes, which were reported to be related to immune response, blood biology, bone remodeling, cancer, etc.In conclusion, this study established a novel PBM proteome database for humans, which improves our understanding of human PBM biology. As a unique resource, this database can serve as a reference map for future in-depth research on PBM and PBM-involved human diseases.