Analysis On Function Of CKL-3Gene T-DNA Insertion Mutant In Arabidopsis

Casein kinase (CK) encompasses a family of serine/threonine kinase. Proteinphosphorylation is the most important regulate protein function in organism. It is aclass of kinase changes activity through protein phosphorylation. Most function of thephosphatase and the relationship between protein phosphorylation and signaltransduction pathways are not clear because of the superposition between genes.Casein kinase I (CKI) isoforms participate in many cellular processes and play key rolein cell development. At present, CKL2were coveraged participate in biological clockregulation in Arabidopsis. Functional analysis of CK1-like protein is fewer, and CKL-3belongs to CK1-like protein gene. This study focused on the expression of CKL-3biological function and the results were as follows：1. In this paper,3216bp genome sequence fragment upstream the start codon ofCKL-3were picked up from www.arabidopsis.org. The promoter cis-acting elementswere analysised by the plantCARE software. The gene chip results showed that ckl-3gene expression in flowers is highest, following by roots, in the leaves and petioles ofweak expression. The gene chip analysis results provided the important reference datafor the following research.2. T-DNA homozygotes were identified by the "double-primer method" and theRT-PCR. RNAs were extracted and gene expression levels were quantified. The resultswere consistent with the prediction of gene chips. We also studied the effect onstress-related gene expression in ABA signal pathway caused by the deletion of CKL-3gene. Mutants are associated to abiotic stress. After different concentrations of ABAconcentration treatments (0.01,0.15, and0.3uM), the results showed mutants are moreinsensitive than the wild type.