Genome-Wide Identification And Evolution Analysis Of The IQD Gene Family In Zea Mays L

Calcium ions took part in a number of physiological responses in the intracellularsecCalcium ions took part in a number of physiological responses in the intracellularsecond messenger in plant cells. the study of Calcium receptor protein binding proteinwill help clarify the properties and functions of CaM signal transduction. In recentyears, the study of the calmodulin-binding protein genes in plants has become a hotissue. In this study,we perform structure analysis, phylogenetic analysis andexpression patterns of IQD gene in maize by bioinformation software.Moreover, theresearch also provided a biological reference for cloning and evolution. The resultsare as follows:1. We perform search, comparison and selection in maize whole genome withvarious bioinformation software, a total of31different IQD genes are found. The IQDgene family of maize are named by the location of its chromosomal and namingmethods of IQD gene family in Arabidopsis. Evolution relationship of IQD typegenes is analysed with construction of phylogenetic tree.Comparative analysis amongIQD gene family of Arabidopsis, sorghum and maize shows that genes of maize andArabidopsis have high degree of homology, suggesting that IQD type gene is a genewith monocot/dicot differences.2. The starting position of IQD type gene in chromosome is determined and itsphysical position of chromosome is mapped. The results show that31IQD genesdistributed in ten chromosomes of maize dispersedly, and chromosome8has thelargest number of7genes; chromosome2,7have the smallest number of1gene,which is consistent with chromosome position of other species reported, and therewas no obvious phenomenon of gene cluster.3. With structure analysis of maize IQD type gene, gene structure of IQD genewhich belongs to one category in evolutionary relationship is similar, and the numberof intron and exon are the same. Homology analysis in31maize IQD genes showsthat the amino acid sequence has high similarity in conserved regions, namely,functional core region, and amino acid composition also has high repeatability.4. Motif analysis shows that IQD type genes have complex structures, whichhas20different kinds of motifs, among which, motif1-6appear in most maize IQDprotein sequences, but motif15,19,20appear less, and genes with these few motifmay play a significant role functionally. 5. With maize EST databases, prediction of specific expression of31maizeIQD gene in different tissues and organs shows that,24genes distributed in7tissuesand organs can express specifically, and4genes can still not be predicted specificexpression in specific tissues and organs. The expression pattern provides animportant theoretical basis for in-depth study of effect and function of IQD genes.