Effects Of0.4mT Power Frequency Magnetic Field On The Cytoskeleton And Motility Associated Signal Proteins Of FL Cell Line

50Hz PFMF (power frequency magnetic field) is one of the most common magnetic field in our environment. The probable effect of PFMF on life is one of the researches most concerned by people. Our previous investigations have indicated that: exposure of0.4mT50Hz PFMF for30min induced EGFR clustered in CHL cell surface and cytoskeleton reorganization in CHL and FL cell lines, and the effects can be totally or partially inhibited by the tyrosine kinase inhibitor PD153035and LTCC inhibitor Nifedipine. More evident showed that activated EGFR and F-actin are associated closely in cells after exposure of0.4mT50Hz PFMF. The association between EGFR and F-actin and the cytoskeleton reorganization contribute to the cell motility. And EGFR signal proteins such as fascin, vinculin, Arp2/3, and MLC play a very important role in cytoskeleton reorganization and cell motility.In order to further illustrate the mechanism of EGFR signaling proteins in the progress of cytoskeleton reorganization and possible motility by PFMF, we studied the bioeffect of0.4mT,50Hz PFMF on the abovementioned signal proteins to explore the effect of PFMF to understand wether and how the PFMF affect the mechanism of cytoskeleton reorganization and probable cell motility by EGFR signal pathway and how the PFMF influenced the related signal proteins, by using techniques as western blotting, immunofluorescence in FL cell line.Under the exposure of0.4mT,50Hz PFMF, it was observed in FL cell line by the method of western blotting that (1) total content of F-actin decreased and ratio of F/G-actin decreased;(2) the amount of fascin ralated with filopodia increased;(3) the amount of vinculin related with adhesion spot increased;(4) the content of Arp3related with lamelipodia increased;(5) content of MLC associated with stress fiber decreased while free MLC content increased compared with the sham; and(6) all abovementioned changes almost recovered by inhibiting LTCC and EGFR tyrosine kinase using NIF and PD. The results indicated that PFMF possibly influenced the cytoskeleton reorganization and motility probably through the interactions between changed level of related proteins and EGFR signal pathway by PFMF.