Synthesis Of 4-amino-8-arylazo-7-oxo-6-phenylpyrazolo [5, 1-c] [1, 2, 4] Triazine-3-carboxylate

Objective It is a method to research anti-cancer drugs that changing the structure of basic groups in the nucleic acid ,and it can restrain the metabolic way by which tumor cells live and reproduce. In this subject, applying bioisoterism, we designed and synthesized a series of purine base analogues, expecting to find lead compounds that have anti-cancer effects by screening them. Methods Ethylcyanoacetate reacted with phenylhydrazine, and coupled with different aromatic diazonium chlorides to give the key intermediates 3-amino-4- arylazo-1-phenyl-5-pyrazolone derivatives. Through diazotization, coupling and intramolecular cyclization, the objective compounds 4-amino-8-arylazo-7-oxo-6- phenylpyrazolo[5,1-c][1,2,4]triazine-3-carboxylate derivatives were synthesized. The structures of these compounds were identified by the comprehensive methods of TLC, LC-MS, 1~H-NMR, IR, UV-Vis and elemental analysis. Result Thirteen new purine analogues were synthesized. Conclusion The resulting compounds were all identical to the objective compounds by spectroscopic methods. The pharmacological activity screening for these compounds is being carried out.