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Polycation Grafted Nanodiamond As A Multifunctional Gene Vector

Posted on:2013-12-17Degree:MasterType:Thesis
Country:ChinaCandidate:P ZhangFull Text:PDF
GTID:2231330362461295Subject:Materials science
Abstract/Summary:PDF Full Text Request
A novel cationic nanodiamond-polymer brush was synthesized by 2-bromoisobutyrate-modified nanodiamond (ND) surface-initiated atomic transfer radical polymerization of 2-(dimethylamino)ethyl methacrylate (DMAEMA). Elemental analysis, FTIR, HRTEM, TGA and zeta potential analysis were used to confirm the successful synthesis of ND-polymer brushes. According to the data obtained by the above characterizations, the polymerization degrees of ND-PDMAEMA-1 and ND-PDMAEMA-2 were calculated to be 6.3 and 74.9, respectively. The ND-brushes were capable of condensing plasmid DNA into stable nanoparticles, with diameters less than 200 nm, as determined by TEM and DLS. Further studies showed that compared with the linear polycation PDMAEMA100, ND-PDMAEMA had a better ability to protect DNA from enzyme degradation, and the ND-PDMAEMA/pDNA complexes were more stable. Transfection studies demonstrated that the ND-brushes could not only efficiently deliver plasmids into COS-7 cells, but also mediate higher expression than PEI25k with lower cytotoxicity. The green fluorescence of ND-brushes could also be detected by laser scanning confocal microscopy, making the nanodiamond-polymer brushes an excellent multifunctional gene vector with not only high transfection efficiency but allowing for bioimaging. We believe that this new type of gene delivery system with dual functions of high transfection efficiency and bioimaging ability will have utility in non-viral gene transfer biomedical applications and pre-clinical studies of cell-based therapies where genetically modified cells can be tracked within the target tissues or organs...
Keywords/Search Tags:Nanodiamond, Polycation, ATRP, Gene transfection, Cell imaging
PDF Full Text Request
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