Effect Of Adding Legume Peptides On Nitrite Formation And Mechanism In Fermented Pickles

In the process of pickles fermentation adding bean peptides, to study theirregulation effects of pickles fermentation. The results showed: in naturalfermentationcan, adding beans peptide can shorten the fermentation cyble24hour, inpure fermentation, adding beans peptide can shorten fermentation cycle48hour, butnitrite content was less afeected. Adding soybean peptide: in natural fermentation,lactobacillus plantarum number increased10~2cfu/mL, bacterial count reduced10cfu/mL; in pure fermentation, lactobacillus plantarum number increased10~3cfu/mL,bacterial count reduced10~2cfu/mL, and nitrite comtent reduced13.56%. Addingmung bean peptide: in natural fermentation, lactobacillus plantarum number increased10cfu/mL, bacterial count reduced less10cfu/mL; in pure fermentation, lactobacillusplantarum number increased10~2cfu/mL, bacterial count reduced10~2cfu/mL, andnitrite comtent reduced12.03%. Adding red bean peptide: in natural fermentation,lactobacillus plantarum number increased10~2cfu/mL, bacterial count reduced10~2cfu/mL; in pure fermentation, lactobacillus plantarum number increased10~3cfu/mL, bacterial count reduced10~3cfu/mL, and nitrite comtent reduced23.33%.To study the change of nitrate reductase enzyme activity and picklesorganization, determine the nitrite forming reason of pickles fermentation. The resultsshowed, the nitrite production of pickles fermentation is caused of the nitratereductase in the bacteria.Through the research of nitrite degradation, to explore the reason of nitrite lowcontent with adding peptide. The results showed, pH value5.5-3.5, lactobacillusplantarum degradation rate of nitrite more than50%; pH value of3the following,acid degradation was advantaged,60hour, pH2.5degration rate of nitrite was50.8%.Adding beans peptide can promote lactobacillus plantarum rapid fermentation,bacterial degradation and acid degradation is more pronounced, meantime beanspeptide is an antioxidant, have hydrogen donor activity, deoxidize nitrite toammonium ions, intensify the effect of nitrite scavenging.